Zoologix performs primate infectious disease tests by PCR for...


African green monkey endogenous virus


B virus


Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi



Chagas' disease

Chikungunya virus

Chlamydia pneumoniae

Chlamydophila trachomatis



Cronobacter sakazakii


Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian


E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species


Epstein-Barr virus


Gibbon ape leukemia


Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster



Lawsonia intracellularis



Lyme disease







Neisseria gonorhoeae

Neisseria meningitidis



Plasmodium species

Reovirus screen

Rhesus rhadinovirus



Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes



Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi



Valley fever

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

Zika virus

* * *

Genetic tests for...

A/B/AB blood type in macaques

Cynomolgus genotyping

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements

Primate genetics: Trim5 alpha PCR test for macaques

primate genetic test data sheet


TRIM5α genotyping for macaques

Test codes:
- detection of Mamu-6 allele by PCR
GP007 - detection of Mamu-7 allele by PCR


The TRIM5α gene has recently been identified as an important factor in restriction of infectivity of immunodeficiency viruses. Homologs of this gene have been identified in humans, nonhuman primates and some other vertebrates.

The functional effect of the TRIM5α protein appears to be somewhat species-specific. For example, TRIM5α restricts SIV infection in Old World monkeys but the human TRIM5α protein does not have a similar restrictive function relative to HIV infection in humans.

The TRIM5α protein possesses a characteristic tripartite structure, its three structural motifs being ring, B Box 2, and coiled coil domains. Together with other members of the TRIM family, TRIM5α also has a C-terminal PRY/SPRY, or B30.2 domain, which is the most important domain conferring antiviral specificity.

Several variants of the TRIM5α protein have been described; they are named Mamu-1 through Mamu-7. In vitro studies have shown that Mamu-1, -2, -3, -4, and -5 can efficiently restrict HIV-1 and MLV-N (N-tropic murine leukemia virus), whereas Mamu-6 does not restrict either retrovirus (Newman et al. 2006).

The Mamu-7 variant, also called TRIMCyp, has recently been discovered in New World owl monkeys and in rhesus macaques. The Mamu-7 variant is created by insertion of a cyclophilin A (CypA) cDNA by retrotransposition into the seventh intron of the TRIM5α gene, leading to replacement of the exon eight-encoded PRY/SPRY/B30.2 domain with the cyclophilin A gene. This change alters antiviral specificity and leads to restriction of retroviruses that recruit CypA to their incoming capsid, including HIV-1, feline immunodeficiency virus and simian immunodeficiency virus from Tantalus monkey (SIVtan).

Characterization of the underlying genotype of the TRIM5α gene is important for the proper selection of macaques for AIDS studies.


  • Help confirm TRIM5α genotype in macaques
  • Prequalification of rhesus and cynomolgus macaques for preclinical trials

Newman, R. M., Hall, L., Connole, M., Chen, G-L., Sato, S., Yuste, E., Diehl, W., Hunter, E., Kaur, A., Miller, G.M. and Johnson, W.E. (2006) Balancing selection and the evolution of functional polymorphism in Old World monkey TRIM5α. PNAS 103: 19134-19139.

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or buccal swab, or 0.2 ml fresh, frozen or fixed tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Normal range: Homozygous or heterozygous for the variant

Turnaround time: 3 business days

Methodology: PCR

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