primate assay data sheet
Monkey parvoviruses: simian, rhesus and pigtailed macaque
parvoviruses
Test code:
S0114 - Ultrasensitive qualitative detection of monkey
parvoviruses by real time PCR. This assay
detects but does not differentiate simian parvovirus,
rhesus macaque parvovirus and pig-tailed macaque parvovirus.
Simian
parvovirus (SPV) was originally isolated during an outbreak of
anemia in cynomolgus macaques. Naturally and experimentally
infected monkeys have many clinical features similar to those of
human B19 infection, such as asymptomatic infection in healthy
animals, anemia in immunocompromised monkeys, and fetal hydrops
in pregnant macaques.
Soon after
the discovery of SPV, two similar outbreaks of anemia in rhesus
and pig-tailed macaques were reported and led to isolation of
two distinct but similar simian parvoviruses (pig-tailed macaque
and rhesus parvovirus).
Like human
B19 virus, SPV contains a short piece of single stranded DNA.
The genomic organization and the protein-coding strategy of SPV
are very similar to those of human parvovirus B19. Because of
these close similarities, SPV can replicate in vitro in both
human and macaque bone marrow mononuclear cells and is cytotoxic
to erythroid progenitor cells. Thus SPV may infect human bone
marrow mononuclear cells in vitro and in vivo and should be
considered a potential zoonosis.
Molecular
detection by PCR of parvovirus carrier status or infection in
monkeys has much higher sensitivity and specificity than
serology (Gallinella
et al., 2003).
Utilities:
-
Help confirm the disease causing agent
-
Help ensure that animal colonies are free of parvoviruses
-
Early prevention of spread of parvoviruses among a
colony
-
Minimize unnecessary occupational exposure to these
viruses
-
Safety monitoring of biological products and vaccines
that derive from monkeys
Reference:
Gallinella, G., Zuffie, E., Gentilomi, G., Manaresi, E.,
Venturoli, S., Bovincini, F., Cricca, M., Zerbini, M. and
Musiani, M. (2003) Relevance of B19 markers in serum samples for
a diagnosis of parvovirus B19-correlated diseases. J Med. Virol.
71:135-139.
Specimen requirement: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml cell culture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
Turnaround time:
2 business days
Methodology:
Qualitative real time polymerase chain reaction.
Normal range:
Nondetected