Zoologix performs primate infectious disease tests by PCR for...


African green monkey endogenous virus


B virus


Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi



Chagas' disease

Chikungunya virus

Chlamydia pneumoniae

Chlamydophila trachomatis



Cronobacter sakazakii


Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian


E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species


Epstein-Barr virus


Gibbon ape leukemia


Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster



Lawsonia intracellularis



Lyme disease







Neisseria gonorhoeae

Neisseria meningitidis



Plasmodium species

Reovirus screen

Rhesus rhadinovirus



Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes



Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi



Valley fever

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

Zika virus

* * *

Genetic tests for...

A/B/AB blood type in macaques

Cynomolgus genotyping

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements

Hepatitis A PCR test for primates
primate assay data sheet

Hepatitis A virus (HAV) by PCR

Test code: S0053
- Ultrasensitive qualitative detection of hepatitis A virus by reverse transcription coupled real time PCR

(A0007 - ELISA detection of total antibodies to hepatitis A virus in nonhuman primates)

Hepatitis A is a contagious liver disease that results from infection with the hepatitis A virus (HAV), a picornavirus. Infected individuals develop symptoms ranging in severity from mild illness lasting a few weeks to severe illness lasting several months. Hepatitis A is usually spread when a person ingests fecal matter from an infected person, through contact with contaminated objects, food, or drinks. Infected persons may develop fever, fatigue, loss of appetite, nausea, vomiting, abdominal pain, dark urine, clay-colored bowel movements, joint pain, and jaundice.

The host range for hepatitis A virus is limited to human and several species of nonhuman primates. Spontaneous hepatitis A infection has been reported to occur in captive non-human primates including the great apes (chimpanzee) as well as Old World (cynomolgus, African vervet, stump-tailed) and New World (aotus) monkeys. The frequent detection of anti-HAV antibody in the sera of newly captured individuals of these species shows that infection with this virus is common in their natural habitat.

Serological diagnosis of hepatitis A infection relies on detecting an elevation of IgM level in patient's blood. However, serology cannot be easily applied to environmental monitoring of contaminated water and sewage samples. Additionally, molecular detection by PCR is highly sensitive and specific (Apaire-Marchais et al., 1994).


  • Help confirm the disease causing agent
  • Help ensure that animal colonies are free of Hepatitis A
  • Early prevention of spread of this virus among a colony
  • Environmental monitoring for this virus
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from primates

Apaire-Marchais, V., Ferre-Aubineau, V., Colonna, F., Dubois, F., Ponge, A., and Billaudel, S. (1994) Development of RT-semi-nested PCR for detection of hepatitis A virus in stool in epidemic conditions. Mol Cell Probes 8:117-124.

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml feces, or 2 rectal swabs, or 0.2 ml serum or plasma, or 0.2 ml fresh or frozen liver tissue, or 0.2 ml water or environmental samples.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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