Need serology?
Yes, we're still the PCR experts. But Zoologix also performs ELISA antibody tests for...

SRV
Herpes B
SIV
STLV
Measles
Hepatitis A
Hepatitis B
Hepatitis C

* * *

Zoologix performs primate infectious disease tests by PCR for...

Adenoviruses

Aspergillus

B virus

Babesia

Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi

Campylobacter

Chagas' disease

Chikungunya virus

Chlamydia pneumoniae

Chlamydophila trachomatis

Clostridium

Cronobacter sakazakii

Cryptosporidium

Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian

Dengue

E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species

Enterovirus

Epstein-Barr virus

Giardia

Gibbon ape leukemia

Helicobacter

Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster

Influenza

Klebsiella

Lawsonia intracellularis

Leptospira

Lyme disease

Lymphocryptovirus

Malaria

Measles

Monkeypox

Monkey parvoviruses

Mycobacteria

Mycoplasma

Neisseria gonorhoeae

Neisseria meningitidis

Papillomavirus

Plasmodium species

Reovirus screen

Rhesus rhadinovirus

Rotavirus

Salmonella

Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes

SV40

SV5

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tuberculosis

Ureaplasma

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

* * *

Genetic tests for...

A/B/AB blood type in macaques

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

smCCR5Δ24
in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements


STLV 1 and 2 PCR test
primate assay data sheet

Simian T-cell lymphotropic/leukemia virus (STLV) types 1 and 2 by PCR

Test codes:
S0014 - Qualitative detection of STLV type 1 by polymerase chain reaction
S0015 - Qualitative detection of STLV type 2 by polymerase chain reaction
P0005 - Ultrasensitive qualitative screen for primate T-cell leukemia/lymphotropic viruses (STLV and HTLV) by real time polymerase chain reaction

(A0005 - ELISA detection of antibodies to simian T-cell lymphotropic virus (STLV) in macaques)

 

The human T-cell leukemia virus (HTLV) is divided into two serologically and genetically distinct types (HTLV-1 and HTLV-2). Both types have a simian relative: HTLV-1 is related to simian T-cell leukemia virus type 1 (STLV-1) and HTLV-2 is related to STLV-2. STLV-1 infects a wide range of wild nonhuman primates (NHPs). Natural infection with STLV-1 can be found among macaques, guenons, mangabeys, baboons, and apes in Asia and Africa. However, STLV-2 has only been identified in captive bonobos (Pan paniscus). The natural occurrence of STLV-2 is still unknown. HTLVs and STLVs are collectively called primate T-cell leukemia virus (PTLV).

For reasons of occupational safety and animal health, as well as to improve the quality of nonhuman primates (NHPs) used in biomedical research, the establishment and maintenance of specific retrovirus-free breeding colonies of NHPs such as macaques are now high priorities. Viruses which have a widespread occurrence and a strong potential for cross-species transmission such as STLV top the priority list. Although virus isolation can be used to diagnose STLV virus infection, it requires a long period of time to obtain results. Furthermore, viral culture is neither sensitive nor specific, and increases the potential risk of laboratory workers contacting this virus. Serological detection of STLV infection is also not very reliable, sensitive or specific. False negative and false positive results occur frequently. A recent study has shown that experimental infection of macaques with STLV-I can be serologically silent for more than 43 months.

STLV detection by PCR is the most rapid, sensitive and specific method for the diagnosis of this infection. The method can also differentiate the virus from other closely related polyomaviruses.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal colonies are free of STLV
  • Early prevention of spread of this virus among a colony
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from primates

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodologies:
S0014
and S0015 - Qualitative PCR
P0005 - Qualitative real time PCR

Normal range: Nondetected

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