Need serology?
Yes, we're still the PCR experts. But Zoologix also performs ELISA antibody tests for...

SRV
Herpes B
SIV
STLV
Measles
Hepatitis A
Hepatitis B
Hepatitis C

* * *

Zoologix performs primate infectious disease tests by PCR for...

Adenoviruses

Aspergillus

B virus

Babesia

Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi

Campylobacter

Chagas' disease

Chikungunya virus

Chlamydia pneumoniae

Chlamydophila trachomatis

Clostridium

Cronobacter sakazakii

Cryptosporidium

Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian

Dengue

E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species

Enterovirus

Epstein-Barr virus

Giardia

Gibbon ape leukemia

Helicobacter

Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster

Influenza

Klebsiella

Lawsonia intracellularis

Leptospira

Lyme disease

Lymphocryptovirus

Malaria

Measles

Monkeypox

Monkey parvoviruses

Mycobacteria

Mycoplasma

Neisseria gonorhoeae

Neisseria meningitidis

Papillomavirus

Plasmodium species

Reovirus screen

Rhesus rhadinovirus

Rotavirus

Salmonella

Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes

SV40

SV5

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tuberculosis

Ureaplasma

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

* * *

Genetic tests for...

A/B/AB blood type in macaques

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

smCCR5Δ24
in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements


Herpes B PCR test
primate assay data sheet

Herpes B virus ("monkey B virus") by PCR

Test codes:

S0078 - Ultrasensitive qualitative detection of herpes B virus by real time polymerase chain reaction

(A0003 - ELISA detection of antibodies to herpes B virus in macaques)

 

Cercopithecine herpesvirus 1 (herpesvirus simiae, herpes B or monkey B virus) is a member of the alphaherpesvirus subfamily and is a common pathogen in macaques. While macaques infected with this virus are usually asymptomatic, zoonosis caused by the virus in human is one of the most deadly acquired infections. Rapid and accurate diagnosis of this virus in macaques can significantly reduce the risk of personnel exposure and prevent spread of the virus through a colony.

Although virus isolation can be used to diagnose B virus infection, a long incubation period is required to obtain results. Viral culture also increases the potential risk of laboratory personnel contacting this level 4 virus. Furthermore, viral culture is less sensitive, reliable and specific than polymerase chain reaction (PCR)-based techniques. False negative and false positive results occur frequently in serologic tests for B virus, and serially collected serological data are often needed to confirm an animal's disease status.

PCR is a rapid, sensitive and specific method for detection of B virus in a sample. The method can differentiate the virus from other closely related primate alphaherpesviruses such as simian agent 8 and herpesvirus papio 2. However, it is important to note that a negative PCR test does not rule out the presence of Herpes B virus, as viremia is intermittent.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal colonies are free of Herpes B virus
  • Early prevention of spread of this virus among a colony
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from monkeys

Specimen requirement:

Preferred samples - Mucosal swab, or 0.2 ml CSF, or 0.2 ml fresh, frozen or fixed CNS tissue.

Less preferred sample - 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

2003-2014 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880