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Yes, we're still the PCR experts. But Zoologix also performs ELISA antibody tests for...

SRV
Herpes B
SIV
STLV
Measles
Hepatitis A
Hepatitis B
Hepatitis C

* * *

Zoologix performs primate infectious disease tests by PCR for...

Adenoviruses

Aspergillus

B virus

Babesia

Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi

Campylobacter

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Chikungunya virus

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Chlamydophila trachomatis

Clostridium

Cronobacter sakazakii

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Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian

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E. coli O157:H7

E. coli panel

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Entamoeba species

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Gibbon ape leukemia

Helicobacter

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Hepatitis B virus

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Herpes ateles

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Herpes tamarinus

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Herpesvirus papio 1 & 2

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Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

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Influenza

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Reovirus screen

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Salmonella

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Simian agent 6 (SA6)

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Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

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Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

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SV40

SV5

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Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tuberculosis

Ureaplasma

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

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Genetic tests for...

A/B/AB blood type in macaques

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

smCCR5Δ24
in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements


Helicobacter PCR tests for primates
primate assay data sheet

Helicobacter species

Test codes:

B0021 - Ultrasensitive qualitative detection of Helicobacter pylori by real time polymerase chain reaction

B0023 - Ultrasensitive qualitative detection of Helicobacter heilmannii by real time polymerase chain reaction

P0010 - Ultrasensitive Helicobacter species screen by real time polymerase chain reaction.  This screen detects but does not differentiate H. pylori, H. heilmannii, H. bilis, H. hepaticus, H. rappini, H. felis, H. salomonis and other Helicobacter species.

P0011 - Ultrasensitive Helicobacter species identification by real time polymerase chain reaction and Restriction Fragment Length Polymorphism.  This 2-stage assay detects and differentiates H. pylori, H. heilmannii, H. bilis, H. hepaticus, H. rappini, H. felis, H. salomonis and other Helicobacter species.

 

Many species of the genus Helicobacter have been identified in mammals and their pathogenicity varies. Some species can induce significant disease while others appear to merely colonize the gastrointestinal tract.

Helicobacter pylori is a gram-negative spiral bacterium found in gastric mucosa and associated with active and chronic gastritis. H. pylori can establish a chronic, persistent infection, which may lead to gastric or duodenual ulcers, gastric cancer and gastric lymphomas. Studies have revealed that approximately 50% of the world’s human population is infected with H. pylori.

Biochemically, the bacterium produces catalase, oxidase and urease enzymes. The urease enzyme permits the bacterium to metabolize urea present in the gastric mucosa and establish a microenvironment favorable to the organism. H. pylori is a highly motile organism with multiple unipolar flagella. Both the urease enzyme and the flagella are considered to be important virulence factors.

Diagnosis of Helicobacter pylori infection in humans relies on upper endoscopy or the 13C-urea breath test (see review by Nakamura, 2001). Although the endoscopy procedure permits culture of the bacterium from biopsy specimens (the gold standard for diagnosis), demonstration of urease activity and histological detection of the germ, the procedure is expensive and invasive. The 13C-urea breath test is a well-established, relatively sensitive, specific and noninvasive method. Molecular tests, such as PCR, can also offer precise diagnosis of H. pylori infections. In fact, molecular testing by PCR can complement other diagnostic tests because it can be applied to archival fixed tissue, environmental samples, gastric fluid, oral secretions, and stool samples, in which traditional diagnostic tests do not have sensitivity and perform poorly. Studies have shown than PCR detection of H. pylori in gastric fluid specimens can reach a sensitivity of 96% and a specificity of 100% (Westblom et al., 1993; Yoshida et al., 1999). This capability is especially useful in monitoring active H. pylori infection in primates and other animals, as the breath test is difficult to conduct for these animals.

Helicobacter heilmannii (previously known as Gastrospirillum hominis) is a 4-10 m long, spiral-shaped, motile bacterium with three to eight coils, a wavelength of about 1 m, up to 14 uni- or bipolar flagella, and no periplasmic filaments. In humans, gastric infection with H. heilmannii is associated with the development of chronic gastritis (found in the stomachs of 0.2 to 4% of patients with gastritis) and low-grade mucosa-associated lymphoid tissue lymphoma in humans. Eradication of H. heilmannii by antibiotic treatment of patients can result in complete remission of MALT lymphoma, indicating a causal relationship between H. heilmannii infection and MALT lymphoma. Unlike H. pylori infections, gastric infections with H. heilmannii or Gastrospirillum-like organisms are not restricted to humans. A broad range of animals, including dogs, cats, pigs, and cattle, are naturally infected, with frequencies ranging from 80% to 100%. It has been suggested that H. heilmannii infection in humans may be a zoonosis and that animals may serve as a reservoir for transmission to humans.

Definitive culture of H. heilmannii has not been achieved to date (Anderson et al., 1996) and diagnosis of H. heilmannii infection is usually made on the basis of its distinct spiral morphology, compared with H. pylori, on silver- stained tissue sections. However, there are a number of large, gastric, spiral organisms such as H. felis, H. salomonis, and H. bizzozeronii which are indistinguishable from H. heilmannii on routine light microscopy, and H. pylori grown in a broth culture can also adopt a morphology identical to that of H. heilmannii (Fawcett et al., 1999). Molecular detection methods, such as PCR, are always required for more definitive identification (Trebesius et al., 2001).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Helicobacter infection
  • Help ensure that animal colonies are free of Helicobacter
  • Early prevention of spread of these bacteria among a colony
  • Minimize personnel exposure to these bacteria
  • Safety monitoring of biological products and vaccines that derive from primates

References:
Nakamura, R.M. (2001) Laboratory tests for the evaluation of Helicobacter pylori infections. J. Clin. Lab. Analysis 15: 301-307.
Westblom, T.U., Phadmis, S., Yang, P. and Czinn, S.J. (1993) Diagnosis of Helicobacter pylori infection by means of a polymerase chain reaction for gastric juice aspirates. Clin. Infect. Dis. 16: 367-371.
Yoshida, H., Maeda, S. and Ogura, K. (1999) PCR-monitoring of gastric juice obtained with the capsulated string for evaluation of H. pylori infection. Nippon Rhinsho 57: 107-110.
Andersen, L.P., Norgaard, A., Holck, S., Blom, J. and Elsborg, L. (1996) Isolation of a "Helicobacter heilmannii"-like organism from the human stomach. Eur. J. Clin. Microbiol. Infect. Dis. 15:95-96.
Fawcett, P.T., Gibney, K.M. and Vinette, K.M. (1999) Helicobacter pylori can be induced to assume the morphology of Helicobacter heilmannii. J. Clin. Microbiol. 37:1045-1048.
Trebesius, K., Adler, K., Vieth, M., Stolte, M. and Haas, R. (2001) Specific detection and prevalence of Helicobacter heilmannii-like organisms in the human gastric mucosa by fluorescent in situ hybridization and partial 16S ribosomal DNA sequencing. J. Clin. Microbiol. 39:1510-1516.

Specimen requirement: 0.2 ml gastric lavage, or rectal swab, or 0.2 ml feces, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodologies:
B0021
:  Qualitative real time PCR
B0023
:  Qualitative real time PCR
P0010:  Qualitative real time PCR
P0011:  Qualitative real time PCR + RFLP

Normal range: Nondetected

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