primate assay data sheet
Lymphocryptoviruses and Epstein-Barr virus
Test codes:
S0032
- Ultrasensitive qualitative detection of Epstein-Barr virus by
real time polymerase chain reaction. This assay detects
only
Epstein Barr virus, not other lymphocryptoviruses.
S0079
- Ultrasensitive qualitative detection of lymphocryptoviruses by
real time polymerase chain reaction. This assay detects
Epstein-Barr and
other lymphocryptoviruses of clinical interest in nonhuman
primates.
The
herpesviridiae family consists of three groups: alpha, beta and
gamma herpesvirus. All three groups have been found in primates
including humans. Old World primates, including great apes, and
New World primates have been studied extensively and found to
harbor several herpesvirus species, most of them gamma
herpesviruses.
The gamma
herpesviruses are grouped into two classes: lymphocryptoviruses
(n1) and rhadinoviruses (n2). The lymphocryptoviruses include
Epstein–Barr virus, lymphocryptovirus of rhesus monkeys, and
Herpesvirus papio of baboons. Rhadinoviruses that infect New
World monkeys include Herpesvirus saimiri, whose natural host is
the squirrel monkey, and Herpesvirus ateles, which infects
spider monkeys. Rhadinoviruses that infect hominoids and Old
World monkeys include Kaposi's sarcoma-associated herpesvirus,
also known as HHV-8, and rhesus monkey rhadinovirus.
LCV strains
isolated from different species of nonhuman primates share a
high degree of similarities, both in genomic sequence and
organization and in biological behavior. Even so, the various
LCV strains each appear to be species specific. For example,
infection of macaques or baboons with B95-8 or P3HR1 EBV strains
fails to trigger any development of EBV pathogenesis. However,
it is important to note that almost all wild caught primates and
many captive ones have been shown to carry LCV antibodies
beginning early in life. Thus, it is possible that the various
LCV strains’ species specificity is actually due to
cross-reactive immunity resulting from prior LCV infection. If
this is the case, then given that many captive primates are
immunosuppressed or infected with immunodeficiency virus,
cross-species infection with different strains of LCV may be
possible in these animals.
Utilities:
-
Help confirm the disease causing agent
-
Shorten the time required to confirm clinical diagnosis
of a lymphocryptovirus infection
-
Help ensure that primate populations are free of
lymphocryptovirus
-
Early prevention of spread of lymphocryptoviruses among
a colony
-
Minimize personnel exposure to this virus
-
Safety monitoring of biological products and vaccines
that derive from monkeys
Specimen requirement: 0.2 ml whole blood in EDTA (purple top) tube.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
Turnaround time:
2 business days
Methodology:
Qualitative real time PCR
Normal range:
Nondetected