primate assay data sheet
Aspergillus
species
Test code:
F0001 - Ultrasensitive qualitative detection of
Aspergillus
fumigatus by real time polymerase chain reaction.
Test code:
F0009 - Ultrasensitive qualitative detection but not differentiation of most
common species of
Aspergillus, such as Aspergillus
fumigatus, A. flavus, A. terreus, A. nidulans,
A. niger and others, by real time polymerase chain reaction
Aspergillus is a filamentous fungus
that occurs ubiquitously in the environment. It is found in
soil, on plants, in decaying organic matter, and in household
dust and building materials. Most people inhale Aspergillus
spores every day without being affected, but for
immunocompromised persons such as AIDS patients and cancer
patients, inhaling the fungal spores may cause illness. The
spectrum of illness includes allergic reactions, lung
infections, and infections in other organs.
There are more than 185 species of
Aspergillus; the most common species are Aspergillus
fumigatus and Aspergillus flavus. Other common species
include Aspergillus terreus, Aspergillus nidulans, and
Aspergillus niger.
Aspergillus
infections in birds, cattle, horses, pigs, primates and other
species have been reported infrequently. Pulmonary symptoms are
often seen in these cases. Healthy unstressed animals are
generally resistant to even high levels of spores. However,
young and old animals, those on antibiotics, and those whose
immune systems are suppressed by surgery, reproduction,
environmental changes, capture, or shipping may become infected.
Since many animals have had prior exposure to
Aspergillus, serological testing is not useful or
reliable. Although traditional culture detection of
Aspergillus is highly specific, culture of this organism is
slow and sensitivity is low. However, molecular detection by
polymerase chain reaction (PCR) is both specific and sensitive,
and is a rapid and effective alternative to other methods (Buess
et al., 2012).
Utilities:
-
Help confirm the disease causing agent
-
Shorten the time required to confirm a clinical
diagnosis of Aspergillus
infection.
-
Help ensure that primate facilities are free of this fungus
-
Early prevention of spread of this fungus
-
Minimize personnel exposure to this fungus
-
Safety monitoring of biological products and vaccines
that derive from susceptible animals
References:
Buess, M., Cathomas, G., Halter, J., Junker, L.,
Grendelmeier, P., Tamm, M. and Stolz, D. (2012) Aspergillus-PCR
in bronchoalveolar lavage for detection of invasive pulmonary
aspergillosis in immunocompromised patients. BMC Infectious
Diseases 12: 237-244.
Specimen requirements:
Throat or
respiratory swab, or 0.2 ml water, or fungal culture, or 0.2 ml
fresh, frozen or fixed tissue, or environmental surface swab.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative
real time PCR
Normal range:
Nondetected
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