Need serology?
Yes, we're still the PCR experts. But Zoologix also performs ELISA antibody tests for...

SRV
Herpes B
SIV
STLV
Measles
Hepatitis A
Hepatitis B
Hepatitis C

* * *

Zoologix performs primate infectious disease tests by PCR for...

B virus

Babesia

Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi

Campylobacter

Chagas' disease

Chlamydia pneumoniae

Chlamydophila trachomatis

Clostridium

Cronobacter sakazakii

Cryptosporidium

Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian

Dengue

Ebola

E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species

Enterovirus

Epstein-Barr virus

Filovirus panel

Giardia

Gibbon ape leukemia

Helicobacter

Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster

Influenza

Klebsiella

Lawsonia intracellularis

Leptospira

Lyme disease

Lymphocryptovirus

Malaria

Marburg virus

Measles

Monkeypox

Monkey parvoviruses

Mycobacteria

Mycoplasma

Neisseria gonorhoeae

Neisseria meningitidis

Papillomavirus

Plasmodium species

Reovirus screen

Rhesus rhadinovirus

Rotavirus

Salmonella

Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes

SV40

SV5

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tuberculosis

Ureaplasma

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

* * *

Genetic tests for...

A/B/AB blood type in macaques

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

smCCR5Δ24
in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements


Simian cytomegalovirus PCR test
primate assay data sheet

Simian cytomegalovirus (SCMV) - also known as simian agent 6 (SA6)

Test code:
S0017 - Ultrasensitive qualitative detection of simian cytomegalovirus (SCMV) by real time polymerase chain reaction

 

Simian cytomegalovirus (SCMV) was first described in 1957 as one of seven viruses isolated from the African green monkey (Cercopithecus aethiops). SCMV, also known as simian agent 6 (SA6) at that time, was first recovered from primary African green monkey kidney cell (AGMK) cultures, giving rise to foci of rounded swollen cells after 2 weeks in culture. Examining the prevalence of SCMV in primary AGMK cells obtained from commercial suppliers indicated that more than 50% of these cultures were positive for the presence of the virus. Neutralizing antibodies to SCMV were found in more than 95% of African green monkey sera studied. Infection of African green monkeys by SCMV appears to be widespread.

The use of primary rhesus macaque (Macaca mulatta) kidney cultures for the production of poliovirus vaccines during the 1950s led to the identification of simian virus 40 (SV40). It was subsequently confirmed that SV40 was a common contaminant of Rhesus monkey culture. Due to the oncogenicity associated with SV40, attempts were made to switch poliovirus vaccine production from rhesus macaque kidney cultures, to ones prepared from African green monkeys. Thus, to ensure a SCMV-free AGMK culture is vital to safe use of poliovirus vaccines.

Although virus isolation can be used to diagnose SCMV infection, a long incubation period is required to obtain results. Viral culture also increases the potential risk of laboratory personnel contacting this virus. Furthermore, viral culture is less sensitive, reliable and specific than newer polymerase chain reaction (PCR)-based techniques.

SCMV detection by PCR is the most rapid, sensitive and specific method for the diagnosis of this infection.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal colonies are free of SCMV
  • Early prevention of spread of this virus among a colony
  • Minimize personnel exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from monkeys

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml serum or plasma.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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