Need serology?
Yes, we're still the PCR experts. But Zoologix also performs ELISA antibody tests for...

SRV
Herpes B
SIV
STLV
Measles
Hepatitis A
Hepatitis B
Hepatitis C

* * *

Zoologix performs primate infectious disease tests by PCR for...

B virus

Babesia

Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi

Campylobacter

Chagas' disease

Chlamydia pneumoniae

Chlamydophila trachomatis

Clostridium

Cronobacter sakazakii

Cryptosporidium

Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian

Dengue

Ebola

E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species

Enterovirus

Epstein-Barr virus

Filovirus panel

Giardia

Gibbon ape leukemia

Helicobacter

Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster

Influenza

Klebsiella

Lawsonia intracellularis

Leptospira

Lyme disease

Lymphocryptovirus

Malaria

Marburg virus

Measles

Monkeypox

Monkey parvoviruses

Mycobacteria

Mycoplasma

Neisseria gonorhoeae

Neisseria meningitidis

Papillomavirus

Plasmodium species

Reovirus screen

Rhesus rhadinovirus

Rotavirus

Salmonella

Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes

SV40

SV5

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tuberculosis

Ureaplasma

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

* * *

Genetic tests for...

A/B/AB blood type in macaques

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

smCCR5Δ24
in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements


Mycoplasma PCR tests for primates

primate assay data sheet

Mycoplasma species

Test codes:

B0002 – Ultrasensitive qualitative detection of Mycoplasma pneumoniae by real time polymerase chain reaction.

B0009 - Ultrasensitive Mycoplasma screen by real time PCR. This screen detects but does not differentiate M. arginini, M. fermentans, M. hominis, M. hyorhinis, M. orale, M. pirum, M. salivarium, M. agassizii, M. cynos and others. This screen does not detect M. pneumoniae or M. pulmonis.

Mycoplasmas are gram-negative bacteria due to their absence of a cell wall. Because of this characteristic, these bacteria are not responsive to many common antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis. There are more than 100 recognized species in the genus Mycoplasma. Some species cause disease in humans, such as M. pneumoniae which causes atypical pneumonia and other respiratory disorders, and M. genitalium which is linked to pelvic inflammatory diseases.

M. pneumoniae was first linked to respiratory infection more than one hundred years ago. M. pneumoniae can be transmitted through close contact via respiratory droplets. M. pneumoniae has a surface receptor which enables attachment to the host cell surface. This receptor can attach to a number of different cell types such as respiratory tract epithelia and red blood cells. At high concentrations, M. pneumoniae can inhibit ciliary action within the respiratory tract and cause cell necrosis. This damage is caused by cytotoxins from M. pneumoniae as well as indirectly from the host immune response.

Many other Mycoplasma species are found in research laboratories as contaminants in cell culture due to contamination from lab staff or contaminated cell culture medium ingredients. Contamination rates by Mycoplasma species in established cell cultures have been reported at 15-35%, with even higher occurrence cited in some populations.  Mycoplasmas may induce cellular changes, including chromosome aberrations and changes in metabolism and cell growth. Severe Mycoplasma infections may even destroy a cell line. Therefore biological products derived from these cultures must be screened routinely to ensure product stability and integrity. A number of techniques have been used to detect Mycoplasma, including DNA probe, enzyme immunoassays, PCR, plating on sensitive agar and staining with a DNA stain including DAPI or Hoechst. Among these, PCR is the most commonly used method due to its high sensitivity and specificity.

Mycoplasma pneumoniae on the other hand is difficult and slow to grow in culture media. However, molecular detection of M. pneumoniae by PCR is highly sensitive and specific, with fast turnaround time, so PCR is useful for detecting this species as well.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Mycoplasma infection.
  • Help ensure that animal populations are free of Mycoplasma species
  • Early prevention of spread of Mycoplasma species
  • Minimize personnel exposure to Mycoplasma species
  • Preservation of cell cultures
  • Safety monitoring of biological products

References:
Armstrong, S.E., Mariano, J.A. and Lundin, D.J. (2010) The scope of Mycoplasma contamination within the biopharmaceutical industry.  Biologicals, 38:211-213.
Maczyńska, B., Matusiewicz, K., Chiciak, J., Stankiewicz, M.,  Sozańska, B. and Boznański, A. (2002) Comparison of detectability of Mycoplasma pneumoniae infections in children, using PCR-test and serological methods: indirect immunofluorescence and immunoenzymatic assay. Clin.  Microbiol. and Infect. 8 (Suppl. 1):  P1346.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml serum, CSF or bronchial lavage, or 0.2 ml fresh, frozen or fixed tissue, or nasopharyngeal swab, throat swab or vaginal swab, or 0.2 ml culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology:
B0002 - Qualitative real time PCR
B0009 - Qualitative real time PCR

Normal range: Nondetected

©2003-2014 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880