Need serology?
Yes, we're still the PCR experts. But Zoologix also performs ELISA antibody tests for...

SRV
Herpes B
SIV
STLV
Measles
Hepatitis A
Hepatitis B
Hepatitis C

* * *

Zoologix performs primate infectious disease tests by PCR for...

Adenoviruses

Aspergillus

B virus

Babesia

Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi

Campylobacter

Chagas' disease

Chikungunya virus

Chlamydia pneumoniae

Chlamydophila trachomatis

Clostridium

Cronobacter sakazakii

Cryptosporidium

Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian

Dengue

E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species

Enterovirus

Epstein-Barr virus

Giardia

Gibbon ape leukemia

Helicobacter

Hepatitis A virus

Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster

Influenza

Klebsiella

Lawsonia intracellularis

Leptospira

Lyme disease

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Malaria

Measles

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Mycobacteria

Mycoplasma

Neisseria gonorhoeae

Neisseria meningitidis

Papillomavirus

Parvoviruses

Plasmodium species

Reovirus screen

Rhesus rhadinovirus

Rotavirus

Salmonella

Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes

SV40

SV5

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tuberculosis

Ureaplasma

West Nile virus (WNV)

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

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Genetic tests for...

A/B/AB blood type in macaques

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

smCCR5Δ24
in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements


Neisseria meningitidis PCR test for primates
primate assay data sheet

Neisseria meningitidis

Test code:
B0014
- Ultrasensitive qualitative detection of Neisseria meningitidis by real time polymerase chain reaction.

 

Neisseria meningitidis is the etiologic agent of meningococcal meningitis. It is a non-spore forming, gram-negative, nonmotile, diplococcal bacterium. These bacteria are aerobic and have fastidious growth requirements, with optimal growth in warm, moist environments. Five N. meningitidis serotypes cause most associated disease worldwide: serotypes circulating in the US are likely to be B, C, or Y, while serotypes A and W-135 are found outside the US.

The natural habitat of N. meningitidis is the human nasopharyngeal tract. Five to fifteen percent of the human population carries the bacteria in its nonpathogenic form. Infection can occur via respiratory droplets, but normally requires close and prolonged exposure, or direct contact with saliva and respiratory secretions. Ill patients or asymptomatic carriers can transmit the organism.

Meningitis is a common symptom of the resultant infection, characterized by fever, severe headache and stiff neck. Patients with meningococcal sepsis may develop high fever, hypotension (low blood pressure), and profound weakness. In either case, patients may develop a characteristic rash including petechiae (pinpoint red spots that do not blanch with pressure) or purpura (purple areas similar to bruises), that are both caused by bleeding into the skin. Purpura fulminans, a hemorrhagic condition causing tissue necrosis and small vessel thrombosis, can result in scarring or limb amputations. Approximately 10-14% of cases of meningococcal disease are fatal. About 11-19% of patients who recover from the infection may develop permanent hearing loss, mental retardation, loss of limbs or other severe sequelae.

No known animal reservoir exists for N. meningitidis; however, experimental infection of nonhuman primates, mice, guinea pigs and rats has been reported.

Culture detection of N. meningitidis has low sensitivity because the organism has fastidious growth requirements. Molecular detection by PCR can detect N. meningitidis with high sensitivity and specificity (Sacchi et al., 2011).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Neisseria meningitidis infection.
  • Help ensure that colonies are free of N. meningitidis
  • Early prevention of spread of N. meningitidis
  • Minimize personnel exposure to N. meningitidis
  • Safety monitoring of biological products

References:
Sacchi, C.T., Fukasawa, L.O., Gonçalves, M.G., Salgado, M.M., Shutt, K.A., Carvalhanas, T.R., Ribeiro, A.F., Kemp, B., Gorla, M.C., Albernaz, R.K., Marques, E.G,, Cruciano, A., Waldman, E.A., Brandileone, M.C., Harrison, L.H,. São Paulo RT-PCR Surveillance Project Team. (2011) Incorporation of real-time PCR into routine public health surveillance of culture negative bacterial meningitis in São Paulo, Brazil. PLoS One. 6:e20675.

Specimen requirements: Nasal swab, or throat swab, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube; or 0.2 ml CSF, or aspirate from skin lesions, or cell culture.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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