Zoologix performs primate infectious disease tests by PCR for...

Adenoviruses

African green monkey endogenous virus

Aspergillus

B virus

Babesia

Baboon endogenous virus

Baylisascaris procyonis

Borrelia burgdorferi

Burkholderia

Campylobacter

Chagas' disease

Chikungunya virus

Chlamydia pneumoniae

Chlamydophila trachomatis

Clostridium

Coccidioides

Cronobacter sakazakii

Cryptosporidium

Cynomolgus polyomavirus

Cytomegalovirus, baboon

Cytomegalovirus, chimpanzee

Cytomegalovirus, human

Cytomegalovirus, macaque

Cytomegalovirus, simian

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E. coli O157:H7

E. coli panel

Encephalitis, Japanese

Encephalitis, St. Louis

Encephalomyocarditis (EMCV)

Entamoeba species

Enterovirus

Epstein-Barr virus

Giardia

Gibbon ape leukemia

Helicobacter

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Hepatitis B virus

Hepatitis C virus

Herpes ateles

Herpes B virus

Herpes simplex type 1

Herpes simplex type 2

Herpes tamarinus

Herpesvirus ateles

Herpesvirus papio 1 & 2

Herpesvirus saimiri

Human adenoviruses

Human herpesviruses types 6, 7 & 8

Human immunodeficiency virus types 1 & 2

Human T cell lymphotropic virus

Human Varicella-Zoster

Influenza type A

Klebsiella

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

Lymphocryptovirus

Malaria

Measles

Monkeypox

Mycobacteria

Mycoplasma

Neisseria gonorhoeae

Neisseria meningitidis

Papillomavirus

Parvoviruses

Plasmodium species

Reovirus screen

Rhesus rhadinovirus

Rotavirus

Salmonella

Shigella and enteroinvasive E. coli

Simian agent 6 (SA6)

Simian agent 8 (SA8)

Simian foamy virus (SFV)

Simian hemorrhagic fever (SHFV)

Simian immunodeficiency virus (SIV)

Simian parainfluenza virus

Simian retrovirus (SRV)

Simian sarcoma virus

Simian T-cell leukemia (STLV) types 1 & 2

Simian T-cell leukemia (STLV) type 3

Simian Varicella-Zoster

Squirrel monkey retrovirus

Streptococcus pneumoniae

Streptococcus pyogenes

SV40

SV5

Syphilis

Tetanus

Toxoplasma gondii

Treponema

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tuberculosis

Ureaplasma

Valley fever

West Nile virus (WNV)

Yaws

Yellow fever

Yersinia pestis

Yersinia pseudotuberculosis

Zika virus

* * *

Genetic tests for...

A/B/AB blood type in macaques

Cynomolgus genotyping

Fetal sexing

Mamu-6 in macaques

Mamu-7 in macaques

CYP2C76 c.449TG>A
in macaques

Mu opioid receptor
in macaques

smCCR5Δ24
in sooty mangabeys

...and more - contact Zoologix with your genetic testing requirements


Cryptosporidium PCR test for primates
primate assay data sheet

Cryptosporidium

Test codes:

P0008 - Ultrasensitive qualitative screen for Cryptosporidium by real time polymerase chain reaction. This screen detects but does not differentiate Cryptosporidium species.

Test P0008 is included in P0041 - waterborne pathogens screening panel

Several of the 13+ species in the Cryptosporidium genus have been confirmed as causative agents of human disease. Cryptosporidium is a parasitic protozoan that is transmitted by multiple routes; the animal host range is diverse. The following Cryptosporidium species are currently accepted, on the basis of host specificity, pathogenesis, morphology and genotyping:

Infecting mammals: Cryptosporidium hominis, C. parvum, C. wrairi, C. felis, C. canis, C. andersoni, C. muris and C. ubiquitum

Infecting birds: C. baileyi, C. meleagridis and C. galli

Infecting reptiles: C. serpentis and C. saurophilum

Infecting fish: C. molnari

Phylogenetic analyses have been largely based on sequencing of the small subunit rRNA gene (18S rRNA), the hsp 70 gene, or other housekeeping or structural genes. These analyses reveal that the various Cryptosporidium species interact in complex ways with hosts. For example, the specific host of C. felis is cats, but this species has also been isolated from a cow, while C. andersoni is morphologically close to C. muris but infects cattle rather than mice. And C. parvum includes a complex of subspecies that specifically infect cattle, pigs, kangaroos, ferrets or monkeys.

With the advance of molecular techniques, knowledge of the epidemiology of human cryptosporidiosis has significantly increased. It has been shown that the vast majority of human cases are caused by C. hominis (synonymous with C. parvum genotype 1) and C. parvum (synonymous with C. parvum genotype 2). Other species, including C. meleagridis, C. felis, C. canis and C. muris can also infect humans and are linked to clinical disease, not only in immunocompromised patients but also in immunocompetent people.

PCR is a rapid and extremely sensitive technique for detection of Cryptosporidium.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Cryptosporidium infection.
  • Help ensure that animal populations are free of Cryptosporidium
  • Early prevention of spread of this protozoan
  • Minimize human exposure to this protozoan

References:
Thomas, A.L. and Chalmers, R.M. (2003) Investigation of the range of Cryptosporidium species detected by commercially available antibody-based tests. Proceedings of the Health Protection Agency Inaugural Conference, Warwick, September.

Specimen requirements: 0.2 ml feces, or rectal swab.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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