We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

AMPKγ3R200Q mutation in pigs

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species


Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli


Cache Valley virus




Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever






Coxiella burnetii



E. coli O157:h7



Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease


Fusobacterium necrophorum

Hepatitis E

Herpes, avian


Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis


Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease


Lumpy skin disease virus


Malignant catarrhal fever (MCF)

Malignant hyperthermia in pigs


Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel



Psittacine beak and feather disease

Psittacine herpes

Q fever



Rift Valley fever virus

Rinderpest virus

RyR1 R615C mutation in pigs


Staphylococcus xylosus

St. Louis encephalitis


Swine malignant hyperthermia


Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus



Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Bovine viral diarrhea PCR test

avian & livestock assay data sheet

Bovine viral diarrhea virus (BVDV)

Test code:
S0141 - Ultrasensitive qualitative detection of bovine viral diarrhea (BVD) virus by reverse transcription coupled real time PCR


Bovine viral diarrhea virus (BVDV) is a single-stranded positive-sense RNA virus classified as a pestivirus belonging to the Flaviviridae family. Infection of cattle with this disease can pose severe economic losses to the dairy and beef industries, primarily due to decreased reproductive performance. BVDV occurs nearly worldwide.

Two major BVDV strains have been identified, designated as genotype 1 or genotype 2 on the basis of antigenic variation and differences in sequences in the 5' UTR of the viral genome. Infection by different strains of BVDV can have different clinical symptoms. Infected cattle may develop gastrointestinal disease, immunosuppression with secondary infections, thrombocytopenia and reproductive failure. In infected pregnant cows, the virus is transmitted to a fetus with a high degree of efficiency, leading to fetal loss, malformations or birth of a persistently infected calf.

Fetal infection with BVDV may result in the birth of a calf immunotolerant to BVDV, with an inapparent persistent infection. These calves can become carriers and remain viremic throughout their lifetimes, shedding infectious BVDV and acting as reservoirs of the virus in cattle herds.

Serology testing is not effective for identifying viremic, immunotolerant calves.  Serology testing is also not very useful for testing pooled serum samples because of its low sensitivity. On the other hand, molecular detection of the virus by PCR is a rapid, specific, sensitive and cost effective method for identifying viremic individuals (Weinstock, et al., 2001).


  • Help confirm the disease causing agent
  • Identify BVD carriers
  • Help ensure that animal herds and populations are free of BVD
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

Specimen requirements: 0.2 ml feces, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml serum, or 0.2 ml tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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