We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

AMPKγ3R200Q mutation in pigs

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species


Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli


Cache Valley virus




Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever






Coxiella burnetii



E. coli O157:h7



Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease


Fusobacterium necrophorum

Hepatitis E

Herpes, avian


Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis


Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease


Lumpy skin disease virus


Malignant catarrhal fever (MCF)

Malignant hyperthermia in pigs


Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel



Psittacine beak and feather disease

Psittacine herpes

Q fever



Rift Valley fever virus

Rinderpest virus

RyR1 R615C mutation in pigs


Staphylococcus xylosus

St. Louis encephalitis


Swine malignant hyperthermia


Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus



Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

avian & livestock assay data sheet


Test code:
S0121 - Ultrasensitive qualitative detection of pseudorabies virus by real time polymerase chain reaction


Pseudorabies is a highly endemic swine disease in most parts of the world. It is caused by porcine herpesvirus 1, which is also called pseudorabies virus (PRV), suid herpesvirus-1 (SHV-1), or Aujeszky's disease virus. In cattle, it is sometimes called mad itch disease virus. PRV is considered the most economically important viral disease of swine in areas where hog cholera has been eradicated.

There are many secondary hosts of pseudorabies, including dogs, cats, cattle, rats and horses, but not humans. Secondary hosts are infected through direct contact with swine, rats, and other infected secondary hosts and by eating infected uncooked pork.

Infected swine are usually asymptomatic, but PRV can cause abortion and high mortality in piglets. In some piglets and mature pigs, coughing, sneezing, fever, constipation, depression, seizures, ataxia, circling, and excess salivation can be observed. Mortality in piglets less than one month of age is close to 100 percent, but it is less than 10 percent in pigs between one and six months of age. In cattle infected with this virus, intense itching can result, followed by neurological signs and death. In dogs, symptoms include intense itching, jaw and pharyngeal paralysis, howling and death. In cats, the disease is so rapidly fatal that there are usually no symptoms.

Although the virus does not infect humans, this virus could pose significant risk to xenotransplantation of organs derived from pigs. Studies in experimental animals have shown that the virus can rapidly spread in the central nervous system.

Detection of the virus can be performed by serology assays, but false positive results have been reported (Jacobs et al., 1999). Molecular detection of viral DNA by PCR is a useful alternative because the method is rapid, sensitive and specific.


  • Help confirm the disease causing agent
  • Identify pseudorabies carriers
  • Help ensure that animal groups and populations are free of pseudorabies virus
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

Jacobs, L., Voets, R. and Bianchi, A.T.J. (1999) Detection of pseudorabies virus DNA in individual single-reactor pigs found in certified pseudorabies-free herds. Res. Vet. Sci. 67: 305-307.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

Pseudorabies PCR test

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