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avian & livestock
assay data sheet
Vesicular stomatitis
virus (VSV)
Test code:
S0068
- Ultrasensitive qualitative detection of vesicular
stomatitis virus by reverse transcription real time polymerase chain
reaction.
This assay
detects and differentiates the New Jersey and Indiana
serotypes of VSV.
Vesicular stomatitis (VS) is a disease of
horses, cattle, swine, sheep, goats and deer caused by related
viruses in the genus Vesiculovirus of the family Rhabdoviridae.
The viral genome is 11 kb and codes for five main proteins:
the nucleocapsid (N), phosphoprotein (P), matrix (M),
glycoprotein(G), and polymerase (L). Serologically, VS viruses
have been classified into two main serotypes: New Jersey (NJ)
and Indiana (IN). In addition to the classic VSV-IN (IN1), two
additional subtypes, Cocal virus (VSV-IN2) and Alagoas virus
(VSV-IN3), have been described.
VS is prevalent in northern South America,
throughout Central America, and at one site in the United
States: Ossabaw Island, Georgia. However, outbreaks of VS have
been reported in the western United States, northern Mexico,
and temperate regions of South America. The disease can cause
blisters on an animal's mouth, teats or hooves. Clinical
outbreaks of VS in livestock can result in significant
economic impact on producers and extensive regulatory
responses by governmental agencies, including trade
restrictions, market closures, and quarantines. Such measures
are needed because of the clinical similarities between VS and
foot-and-mouth disease and other foreign vesicular diseases in
livestock species.
Utilities:
- Confirm the disease causing agent
- Distinguish between VS and other
vesicular diseases including foot-and-mouth
- Shorten the time required to confirm a
clinical diagnosis of VS infection
- Ensure that animal herds and populations
are free of VS
- Early prevention of spread of VS
- Minimize human exposure to the virus
References:
Magnuson, R.J., Triantis, J., Rodriguez, L.L., Perkins, A.,
Meredith, C.O., Beaty, B., McCluskey, B., and Salman M. (2003)
A single-tube multiplex reverse transcription-polymerase chain
reaction for detection and differentiation of vesicular
stomatitis Indiana 1 and New Jersey viruses in insects. J.
Vet. Diagn. Invest. 15:561-567.
Specimen requirements: 1
ml whole blood in EDTA (purple top) or ACD (yellow top) tube,
or lesion swab,
or nasopharyngeal swab,
or 0.5 ml
nasal wash, or 0.5 ml tissue,
shipped overnight at room temperature; or
0.5 ml tissue, shipped
frozen.
For specimen types other than those listed
here, please call to confirm specimen acceptability and
shipping instructions.
For all specimen types, if there will be a
delay in shipping, or during very warm weather, refrigerate
specimens until shipped and ship with a cold pack unless more
stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit.
See shipping instructions for
more information.
Turnaround time: 2 business
days
Methodology: Qualitative
reverse transcription real time PCR
Normal range: Nondetected
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