avian & livestock assay data sheet
Vesicular stomatitis virus (VSV)
S0068 - Ultrasensitive qualitative detection of vesicular
stomatitis virus by reverse transcription
coupled real time polymerase chain reaction.
This assay detects and differentiates the New
Jersey and Indiana serotypes of VSV.
Vesicular stomatitis (VS) is a disease of
horses, cattle, swine, sheep, goats and deer
caused by related viruses in the genus
Vesiculovirus of the family Rhabdoviridae. The
viral genome is 11 kb and codes for five main
proteins: the nucleocapsid (N), phosphoprotein
(P), matrix (M), glycoprotein(G), and polymerase
(L). Serologically, VS viruses have been
classified into two main serotypes: New Jersey
(NJ) and Indiana (IN). In addition to the
classic VSV-IN (IN1), two additional subtypes,
Cocal virus (VSV-IN2) and Alagoas virus
(VSV-IN3), have been described.
VS is prevalent in northern South America,
throughout Central America, and at one site in
the United States: Ossabaw Island, Georgia.
However, outbreaks of VS have been reported in
the western United States, northern Mexico, and
temperate regions of South America. The disease
can cause blisters on an animal's mouth, teats
or hooves. Clinical outbreaks of VS in livestock
can result in significant economic impact on
producers and extensive regulatory responses by
governmental agencies, including trade
restrictions, market closures, and quarantines.
Such measures are needed because of the clinical
similarities between VS and foot-and-mouth
disease and other foreign vesicular diseases in
Help confirm the disease causing agent
Distinguish between VS and other vesicular diseases
Shorten the time required to confirm a clinical
diagnosis of VS infection
Help ensure that animal herds and populations are free of VS
Early prevention of spread of VS
Minimize human exposure to the virus
Magnuson, R.J., Triantis, J., Rodriguez, L.L.,
Perkins, A., Meredith, C.O., Beaty, B.,
McCluskey, B., and Salman M. (2003) A
single-tube multiplex reverse
transcription-polymerase chain reaction for
detection and differentiation of vesicular
stomatitis Indiana 1 and New Jersey viruses in
insects. J. Vet. Diagn. Invest. 15:561-567.
Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD
(yellow top) tube, or lesion swab or
nasopharyngeal swab, or 0.2 ml nasal wash, or 0.2 ml fresh or frozen tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay
in shipping, or during very warm weather,
refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens
should be shipped so as to remain frozen in
transit. See shipping
instructions for more information.
2 business days
Qualitative reverse transcription coupled real