avian & livestock assay data sheet
- Ultrasensitive qualitative detection of bovine parvovirus by
real time polymerase chain reaction
Bovine parvovirus (BPV), also known as
haemadsorbing enteric virus, is a member of the parvovirus group.
There are three major species, BPV1, 2 and 3. The virus is a
single stranded DNA virus with a genome of about 5.5 kb, which
encodes seven major proteins.
BPV most commonly causes
diarrhea in neonatal calves and respiratory and reproductive
disease in adult cattle. Reproductive infection causes abortion
and the birth of weak or stillborn calves. Respiratory signs
include coughing, dyspnea and nasal discharge.
Serology has been used to diagnose infection of cattle by
this virus. However, since many cattle have been exposed to this
virus (Sandals et al., 1995), serologic methods may not be
sufficient for rapid diagnosis of recent exposure. Molecular
detection by PCR enables rapid, highly specific and sensitive
detection of the virus.
Help confirm the disease causing agent
Identify bovine parvovirus carriers
Help ensure that animal groups and populations are free of
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive from
Povey, R.C. and
Meek, A. H. (1995)
Prevalence of bovine parvovirus infection in Ontario dairy cattle.
Can. J. Vet. Res. 59: 81–86.
Specimen requirements: 0.2 ml whole blood
in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml feces,
or 0.2 ml fresh, frozen or fixed tissue, or nasal swab, or fecal
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
Turnaround time: 2 business days
Methodology: Qualitative real time PCR
Normal range: Nondetected
Bovine parvovirus PCR test