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avian & livestock assay data sheet
Bovine papular stomatitis virus (BPSV)
Test code:
S0202
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Ultrasensitive
qualitative detection of bovine papular stomatitis virus by
real time PCR
Bovine papular
stomatitis virus (BPSV) is a parapoxvirus associated with
papular and erosive lesions on the muzzle, lips, oral mucosa and
occasionally teats of cattle. Infected calves usually show more
severe symptoms than older cattle. BPSV is usually spread by
direct contact with infected cattle or saliva from an infected
animal. People can be infected through close contact with
infected cows; dairy farm workers sometimes become infected by
handling lesions of an infected animal without gloves, allowing
the virus to enter through broken skin or abrasions.
BPSV infection
in humans usually results in proliferative lesions characterized
by dermal edema and scab formation. Sometimes lesions are
characterized by large nodules on the hands or face.
Clinically, BPSV-infected cattle have symptoms
similar to those of foot-and-mouth disease. It is important that
the two diseases are positively differentiated when symptoms are
seen, because foot-and-mouth disease is highly contagious and
can have very serious impacts on livestock over wide areas,
requiring drastic control measures.
Traditional serological detection of antibodies
to BPSV may not be suitable to confirm the diagnosis of BPSV
infection because of cross-reactivity to antibodies to other
viruses, such as lumpy skin disease virus and pseudocowpox
virus. However, molecular detection by polymerase chain reaction
(PCR) is highly specific, sensitive and rapid, and is a good
alternative to more traditional methods (Inoshima et al., 2000).
Utilities:
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Help confirm the disease causing agent
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Identify BPSV carriers
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Help ensure that animal herds and populations are free of BPSV
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Early prevention of spread of this virus among animals
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Minimize human exposure to this virus
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Safety monitoring of biological products that derive
from animals
References:
Inoshima, Y., Morooka, A. and Sentsui,
H. (2000) Detection and diagnosis of parapoxvirus by the
polymerase chain reaction. J. Virol. Methods. 84:201-208.
Specimen requirements:
Lesion swab, or
0.2 ml saliva, or 0.2 ml whole blood in EDTA (purple top) tube.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
Turnaround time:
2 business days
Methodology:
Qualitative reverse transcription coupled real time PCR
Normal range:
Nondetected
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