We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

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Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

AMPKγ3R200Q mutation in pigs

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Aujeszky's disease

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Avibacterium paragallinarum

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea type 1

Brachyspira pilosicoli

Brucella

Cache Valley virus

Camelpox

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydia/Chlamydophila genus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coccidiodes

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

Ebola Reston

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowl adenovirus

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza type A

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Lawsonia intracellularis

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine hemorrhagic enteropathy

Porcine intestinal adenomatosis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

RyR1 R615C mutation in pigs

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Taenia solium

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichinella spiralis

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

avian & livestock assay data sheet

Bovine rhinoviruses

Test codes:
S0181
- Ultrasensitive qualitative detection of group A bovine rhinoviruses by reverse transcription coupled real time polymerase chain reaction
S0182
- Ultrasensitive qualitative detection of group B bovine rhinoviruses by reverse transcription coupled real time polymerase chain reaction

Rhinoviruses belong to the family picornaviridae. Picornaviruses are antigenically diverse, small, non-enveloped viruses with a positive strand RNA genome. Rhinoviruses are the largest genus within this family. They primarily infect nose and throat of humans and animals. Cattle infected with rhinoviruses can develop subclinical or clinical symptoms depending on the health status of the animal. Rhinoviruses have been found in the upper respiratory tract of animals with acute respiratory disease and in nasal swabs from apparent healthy animals.

Two groups of bovine rhinoviruses have been reported: group A virus was formerly known as bovine rhinoviruses 1 & 3, whereas group B virus was formerly known as bovine rhinovirus 2. These two groups of bovine rhinoviruses are similar in terms of their disease spectrums.

Serological detection of these viruses is not very useful for diagnostic purposes because most animals have been previously exposed and carry circulating antibodies. Antigen detection of these viruses has low sensitivity. However, molecular detection of these viruses by polymerase chain reaction (PCR) is highly specific and sensitive, and is a good alternative to traditional methods.

Utilities:

  • Help confirm the disease causing agent
  • Identify bovine rhinovirus carriers
  • Help ensure that herds and animal populations are free of these viruses
  • Early prevention of spread of these viruses among animals
  • Minimize human exposure to these viruses
  • Safety monitoring of biological products that derive from animals

References:
Do, D. H., Laus, S., Leber, A., Marcon, M. J., Jordan, J.A., Martin, J.M., and Wadowsky, R, M. (2010) A one-step, real-time PCR assay for rapid detection of rhinovirus. J. Mol. Diagn. 12: 102–108.

Specimen requirements: Nasal swab, or throat swab, or 0.2 ml culture.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative one-step reverse transcription coupled real time PCR

Normal range: Nondetected

Bovine rhinovirus PCR test

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