avian & livestock assay data sheet
Peste des petits ruminants virus (PPRV)
- Ultrasensitive qualitative detection of peste des petits
ruminants virus (PPRV) by reverse transcription coupled real time PCR.
Peste des petits ruminants (PPR), also known as
'goat plague’, is a viral disease of goats and sheep. It is
caused by a morbillivirus in the paramyxovirus family, related
to the rinderpest, measles and canine distemper viruses.
Infected animals develop fever, sores in the mouth, diarrhea and
pneumonia; the disease is sometimes fatal. Cattle and several
species of wild ruminants have been infected experimentally, but
goats and sheep are the usual targets
Infected animals can secrete the virus in eye and
nasal discharge, secretions from coughing, and feces. Close
contact between animals result in inhalation of fine droplets
that are released into the air when the infected animals cough
and sneeze. Water, feed troughs and bedding can also be
contaminated with secretions and become additional sources of
infection. Since animals can shed the virus before showing
symptoms, it can be difficult to control the disease unless
proactive testing is done
Culture detection of this virus is slow, and
serological methods for diagnosing this virus can produce false
positive results due to cross-reactivity with antibodies of
other closely related viruses. Molecular detection by PCR is
rapid, highly sensitive and specific (Sharawi et al., 2010).
Help confirm the disease causing agent
Identify PPRV carriers
Screen research materials for the presence of PPRV
Help ensure that animal groups are free of PPRV
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive from
Sharawi, S.S., Yousef, M.R., Al-Hofufy, A.N. and Al-Blowi, M.H.
(2010) Isolation, serological and real time PCR diagnosis of
Peste des petites ruminants virus in naturally exposed Arabian
gazelle in Saudi Arabia.
Vet. World 3:489-494.
Nasal swabs, or environmental swabs, or 0.2 ml whole blood in EDTA (purple top) or ACD
(yellow top) tube, or 0.2 ml feces, or 0.2 ml culture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
for more information.
Turnaround time: 2 business days
Methodology: Qualitative reverse
transcription coupled real time PCR