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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

AMPKγ3R200Q mutation in pigs

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Aujeszky's disease

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Avibacterium paragallinarum

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea type 1

Brachyspira pilosicoli

Brucella

Cache Valley virus

Camelpox

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydia/Chlamydophila genus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coccidiodes

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

Ebola Reston

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowl adenovirus

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza type A

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Lawsonia intracellularis

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine hemorrhagic enteropathy

Porcine intestinal adenomatosis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

RyR1 R615C mutation in pigs

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Taenia solium

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichinella spiralis

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Peste des petits ruminants virus (PPRV) PCR test
avian & livestock assay data sheet

Peste des petits ruminants virus (PPRV) NOTE: THIS TEST IS NOT PERFORMED ON SAMPLES TAKEN FROM ANIMALS OWNED OR LOCATED IN THE STATE OF CALIFORNIA.

Test code: S0200 - Ultrasensitive qualitative detection of peste des petits ruminants virus (PPRV) by reverse transcription coupled real time PCR.

Peste des petits ruminants (PPR), also known as 'goat plague’, is a viral disease of goats and sheep. It is caused by a morbillivirus in the paramyxovirus family, related to the rinderpest, measles and canine distemper viruses. Infected animals develop fever, sores in the mouth, diarrhea and pneumonia; the disease is sometimes fatal. Cattle and several species of wild ruminants have been infected experimentally, but goats and sheep are the usual targets.

Infected animals can secrete the virus in eye and nasal discharge, secretions from coughing, and feces. Close contact between animals result in inhalation of fine droplets that are released into the air when the infected animals cough and sneeze. Water, feed troughs and bedding can also be contaminated with secretions and become additional sources of infection. Since animals can shed the virus before showing symptoms, it can be difficult to control the disease unless proactive testing is done.

Culture detection of this virus is slow, and serological methods for diagnosing this virus can produce false positive results due to cross-reactivity with antibodies of other closely related viruses. Molecular detection by PCR is rapid, highly sensitive and specific (Sharawi et al., 2010).

Utilities:            

  • Help confirm the disease causing agent
  • Identify PPRV carriers
  • Screen research materials for the presence of PPRV
  • Help ensure that animal groups are free of PPRV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from susceptible animals

References:
Sharawi, S.S., Yousef, M.R., Al-Hofufy, A.N. and Al-Blowi, M.H. (2010) Isolation, serological and real time PCR diagnosis of Peste des petites ruminants virus in naturally exposed Arabian gazelle in Saudi Arabia.
Vet. World 3:489-494.

Specimen requirements: Nasal swabs, or environmental swabs, or 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml feces, or 0.2 ml culture.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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