& livestock assay data sheet
- Ultrasensitive detection of Rinderpest virus by reverse transcription
coupled real time PCR
Rinderpest virus (RPV) is an RNA virus belonging to the genus
Morbillivirus of the
family Paramyxoviridae. Although there is only one serotype of
RPV, different strains of this virus vary greatly in their
pathogenicity, from those causing 100% mortality, as seen with
the Saudi/81 strain, to others in which infection causes no
detectable clinical signs.
Rinderpest disease, also known as cattle plague, is a contagious viral
disease affecting clovenhoofed animals, mainly cattle and
buffalo. Many species of wild and domestic cloven-hoofed
animals, including sheep and goats, only develop mild symptoms
when infected, but the mortality rate can reach up to 100 per
cent in highly susceptible cattle or buffalo herds. Infected
animals can develop fever, necrotic stomatitis, gastroenteritis
and lymphoid necrosis.
Transmission of the virus requires close contact between animals. The
virus is present in small amounts of nasal and ocular secretions
1–2 days before fever begins; levels becomes high in secretions
and excretions during the first week of clinical disease and
decrease rapidly as animals develop specific antibodies and
begin to recover.
pigs may remain chronically infected and excrete the virus for 6
weeks after infection. Contaminated pens and garbage feeding,
particularly with material from international airports or
seaports, are documented methods of spread due to the resistance
of the virus to inactivation.
Laboratory tests are important to differentiate the disease from bovine
viral diarrhea in particular, as well as East Coast fever,
foot-and-mouth disease, infectious bovine rhinotracheitis, and
malignant catarrhal fever. Viral isolation and detection of
specific viral antigens in affected tissues using an
immunodiffusion test was formerly the standard, but simpler,
more rapid and more discriminating tests, such as immune capture
ELISA and reverse transcription coupled real time PCR, are
increasingly being used as the first line of diagnosis.
(Carrillo et al., 2010).
Help confirm the disease causing agent
Identify Rinderpest virus carriers
Help ensure that animal herds and populations are free of
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive
Carrillo, C., Prarat, M., Vagnozzi, A., Calahan, J.D., Smoliga, G.,
Nelson, W.M. and Rodriguez, L.L. (2010) Specific detection of
Rinderpest virus by real-time reverse transcription-PCR in
preclinical and clinical samples from experimentally infected
cattle. J. Clin. Microbiol. 48:4094-4101.
0.2 ml whole
blood in EDTA (purple top) or ACD (yellow top) tube,
or nasal swab,
or 0.2 ml feces, or rectal swab, or 0.2 ml fresh or frozen
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
for more information.
2 business days
Qualitative reverse transcription coupled real time PCR