avian & livestock assay data sheet
transmissible gastroenteritis virus (TGEV)
- Ultrasensitive qualitative detection of porcine transmissible
gastroenteritis virus by reverse transcription coupled real time
polymerase chain reaction
Transmissible gastroenteritis (TGE) is an enteric disease of
pigs. TGE virus (TGEV) is an RNA virus belonging to the genus
Coronavirus of the family Coronaviridae. The virus is very
stable when stored frozen, but labile at room temperature or
above. It is also vulnerable to sunlight and oxidizing
disinfectants such as bleach or iodine solution.
The major route of transmission of TGEV is fecal-oral. Once pigs
are infected, the incubation period varies from 18 hours to 3
days. The virus multiplies in the intestines and damages
enterocytes lining the small intestine, producing villous
atrophy and enteritis. Major symptoms include diarrhea and
vomiting. The mortality rate is high in neonates.
Extra-intestinal sites of virus multiplication include the
respiratory tract and mammary tissues, but the virus is most
readily isolated from the intestinal tract and from feces.
It has been suggested that wild and domestic carnivores, such as
foxes, dogs, and possibly mink and cats, serve as carriers of
the virus and contribute to seasonal epidemics. However only
virus excreted by serially TGEV-infected dogs has been confirmed
as infectious for pigs.
Diagnosis by viral culture is time consuming and is not a very
sensitive means of detection of this virus. Diagnosis by
serological detection has the risk of cross reactivity with
other similar viruses, such as porcine respiratory coronavirus.
Molecular detection by PCR can provide rapid, sensitive and
specific detection of the virus (Paton et al., 1997).
Help confirm the disease causing agent
Identify porcine TGEV carriers
Help ensure that animal groups and populations are free of
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive from
D., Ibata, G., Sands, J. and McGoldrick,
Detection of transmissible gastroenteritis virus by RT-PCR and
differentiation from porcine respiratory coronavirus. J.
Virol. Methods. 66:303-309.
Specimen requirements: 0.2 ml whole blood
in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml feces,
or 0.2 ml fresh, frozen or fixed tissue, or nasal swab, or fecal
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
Turnaround time: 2 business days
Methodology: Qualitative reverse
transcription coupled real time PCR
Normal range: Nondetected
Porcine TGEV PCR test