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avian & livestock assay data sheet
Yersinia pseudotuberculosis
Test code:
B0062 - Ultrasensitive qualitative detection of
Yersinia
pseudotuberculosis by real time
polymerase chain reaction
Yersinia pseudotuberculosis is a gram-negative bacterium which primarily causes disease
in animals, although humans may also be infected through food
contamination. Infection with this pathogen
can cause tuberculosis-like symptoms,
including localized tissue necrosis and granulomas in the
spleen, liver, and lymph node.
The bacteria
occur widely in various host species, including dogs, cats,
horses, cattle, rabbits, deer, rodents, and birds. Documented
cases of occupational exposure to
Y. pseudotuberculosis include butchers working in abattoirs
slaughtering swine. Outbreaks of
Y. pseudotuberculosis
in animal facilities have occasionally been reported, including
in primate facilities (Bronson et al., 1972; Iwata et al.,
2008).
Successfully
culturing Y.
pseudotuberculosis is very difficult, and serologic tests in
many cases require serial testing on multiple serum samples
taken at several time points. Molecular detection by PCR offers
a rapid, sensitive and specific method for detecting
Y. pseudotuberculosis.
Utilities:
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Confirm the disease causing agent
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Ensure that flocks are free of
Yersinia pseudotuberculosis
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Early prevention of spread of this bacterium among a
flock
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Minimize human exposure to disease-causing bacteria
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Safety monitoring of biological products and vaccines
that derive from birds
References:
Bronson, R.T., May, B.D. and Ruebner, B.H. (1972) An Outbreak of
Infection by Yersinia pseudotuberculosis in Nonhuman Primates. Am. J. Pathol. 69:
289–308.
Iwata, T., Une, Y., Okatani, A.T., Kato, Y., Nakadai,
A., Lee, K., Watanabe, M., Taniguchi, T., Elhelaly, A.E., Hirota,
Y. and Hayashidani, H. (2008) Virulence characteristics of
Yersinia pseudotuberculosis isolated from breeding monkeys in
Japan. Vet Microbiol. 129:404-409.
Specimen requirements:
Rectal swab, or cloacal swab, or 0.5 ml feces, or 0.5 ml fresh,
frozen or fixed tissue, or 0.5 ml whole blood in EDTA (purple
top) or ACD (yellow top) tube (all volumes 0.2 ml for avian).
For specimen
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for more information.
Turnaround time:
2 business days
Methodology:
Qualitative real time PCR
Normal range:
Nondetected
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