avian & livestock assay data sheet
Caprine arthritis-encephalitis (CAE) virus
Test code:
S0151
- Ultrasensitive detection of caprine arthritis-encephalitis (CAE) virus
by real time PCR
Caprine
arthritis-encephalitis
virus (CAEV) is a lentivirus that can infect goats,
causing chronic disease of the joints and on rare occasions
encephalitis in young goats. CAEV infects and replicates within
white blood cells and therefore any bodily secretions which
contain white blood cells are potential sources of virus to
other goats in the herd. However, not all goats that are
infected with CAEV will develop the disease, and these
asymptomatic carriers may spread the disease within the herd. It
is thus important to test goats routinely for infection.
Serology
testing is routinely used to test goats for past exposure to
this virus. However, a positive serology result cannot confirm
current status, and the presence of the antibodies may not
completely protect a goat from infections. More than 90% of
serology positive goats may be free of any clinical signs but
remain potentially infectious. On the other hand, a negative
serology result does not definitively rule out infection,
because goats recently infected with CAEV may have antibody
titers too low to be reliably detected by serology assays. PCR
detection of the virus is a rapid, specific and sensitively
method for detecting the presence of the virus in a sample. PCR
testing can also be performed on samples other than serum, such
as semen, milk or other bodily secretions (Wagner et al., 2004).
Utilities:
-
Help confirm the disease causing agent
-
Identify CAE virus carriers
-
Help ensure that goat herds are free of CAE virus
-
Early prevention of spread of the virus among animals
-
Minimize human exposure to the virus
-
Safety monitoring of biological products that derive
from goats
References:
Wagner, L.H.A.,
Jansen, A., Bleumink-Pluym, N.M.C., Lenstra, J.A. and Houwers,
D.J. (2004) PCR detection of lentiviral GAG segment DNA in the
white blood cells of sheep and goats. Vet. Res. Comm. 22:
355-362.
Specimen requirements:
0.2 ml
whole blood in EDTA (purple top) tube, or 0.2 ml milk
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative real time PCR
Normal range:
Nondetected
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