We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

AMPKγ3R200Q mutation in pigs

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species


Aujeszky's disease

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Avibacterium paragallinarum

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea type 1

Brachyspira pilosicoli


Cache Valley virus




Caprine arthritis-encephalitis (CAE) virus

Chlamydia/Chlamydophila genus

Chlamydophila psittaci

Classical swine fever






Coxiella burnetii



Ebola Reston

E. coli O157:h7



Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowl adenovirus


Fusobacterium necrophorum

Hepatitis E

Herpes, avian


Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza type A

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Lawsonia intracellularis


Lumpy skin disease virus


Malignant catarrhal fever (MCF)


Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine hemorrhagic enteropathy

Porcine intestinal adenomatosis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel



Psittacine beak and feather disease

Psittacine herpes

Q fever



Rift Valley fever virus

Rinderpest virus

RyR1 R615C mutation in pigs


Staphylococcus xylosus

St. Louis encephalitis



Swine vesicular disease

Taenia solium

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichinella spiralis



Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

avian & livestock assay data sheet

Porcine endogenous retrovirus (PERV)

Test code:
- Ultrasensitive qualitative detection of replication-competent porcine endogenous retrovirus by reverse transcription coupled real time PCR.  This assay detects but does not differentiate types A and C porcine endogenous retroviruses.

Test code: S0230 - Ultrasensitive qualitative detection of porcine endogenous viral DNA type C by real time PCR. This assay detects type C only.


Porcine endogenous retrovirus (PERV) belongs to the gamma-retrovirus genus of the family retroviridae; the virus is enveloped and icosahedral in shape. The viral genome consists of a linear positive-sense single-stranded RNA, approximately 8.0-8.5 kb in length.

In recent years, xenotransplantation involving the movement of porcine-derived tissues into human has increasingly been gaining attention. However, xenotransplantation may be associated with the risk of transmission of zoonotic agents. In pigs, porcine endogenous retroviruses (PERVs) pose a potentially high risk because they are integrated into the genome of all pigs. Although these PERVs can infect human cells in vitro, PERV transmission from transplanted pig tissues to human or primate recipients has never been demonstrated. In addition, inoculation of immunosuppressed small animals and non-human primates failed to result in demonstrable PERV infection. Even though no diseases have been associated with these viruses, routine screening for expression of these dormant retroviruses is suggested (Scobie and Takeuchi, 2009).

Detection of reactivation of replication-competent endogenous viral particles in samples of porcine origin relies on the elimination of contamination from porcine host cell genomic DNA prior to reverse transcription and PCR.

Detection of porcine endogenous retrovirus can be performed by viral culture, but culture sensitivity is low. Molecular detection by polymerase chain reaction (PCR) is highly sensitive, specific and rapid.


  • Monitor PERV expression in pigs
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from pigs

Scobie, L. and Takeuchi, Y. (2009) Porcine endogenous retrovirus and other viruses in xenotransplantation. Curr. Opin. Organ Transplant. 14:175-179.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml fresh, frozen or fixed tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative one-step reverse transcription coupled real time PCR

Normal range: Nondetected


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