avian & livestock assay data sheet
Porcine enterovirus (PEV 8, 9 and 10)
Ultrasensitive detection but not differentiation of porcine
enteroviruses (PEV 8, 9 and 10) by reverse transcription
Porcine enteroviruses are porcine picornaviruses and are ubiquitous in
nature. Porcine enteroviruses (PEV) formerly comprised at least
13 serotypes, grouped into three species. However, porcine
enterovirus 1 (PEV 1, the prototype “Teschen virus” and “Talfan
virus”), PEV 2 through 7, and PEV 11-13 have now been moved to a
new genus called
Teschovirus. Porcine enteroviruses 8-10 remain in the
original Enterovirus genus.
Infection with these viruses can sometimes cause neurological disorders,
fertility disorders, and dermal lesions of swine. However, most
infections are asymptomatic.
Infected pigs can shed the viruses in feces. Spread of the virus is
usually by direct or indirect contact with infected pigs, and
transmission is usually through the fecal-oral route. Infected
fetuses born alive are sometimes a source of viral exposure of
other pigs. Fomites can also carry the virus, which is
relatively stable in the environment. All ages of swine are
susceptible if they have not previously encountered a
picornavirus of that serogroup.
Serological detection of infection requires paired serum samples taken at
least two weeks apart to show a rising titer in serum
neutralization or complement fixation tests. Virus isolation in
tissue culture and identification by immunofluorescence or
identification of viral antigen in tissue by similar technique
may be useful. However, these techniques are not available in
all diagnostic laboratories in the United States because
virulent porcine picornaviral infections seldom occur there.
Secondly, those techniques are not very sensitive and are
relatively slow. Molecular detection by PCR, on the other hand,
is increasingly the method of choice because of its sensitivity,
specificity and faster turnaround time (La Rosa et al., 2006).
Help confirm the disease causing agent
Identify PEV carriers
Help ensure that facilities and populations are free of
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive
La Rosa, G., Muscillo, M., Di Grazia, A., Fontana, S., Iaconelli, M. and
Tollis, M. (2006) Validation of rt-PCR assays for molecular
characterization of porcine teschoviruses and enteroviruses. J.
Vet. Med. B. Infect. Dis. Vet. Public Health 53:257-265.
Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml feces, or fecal or rectal swabs, or 0.2 ml
urine, or 0.2 ml cerebrospinal fluid, or 0.2 ml fresh or frozen tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative reverse transcription real time PCR