avian & livestock assay data sheet
Bovine respiratory syncytial virus (BRSV)
- Ultrasensitive detection of bovine respiratory syncytial
virus by reverse transcription coupled real time PCR.
Bovine respiratory syncytial virus (BRSV) is
an RNA virus belonging to the paramyxoviridiae family. This
virus is named for its characteristic cytopathic effect on cell
culture, the formation of syncytial cells.
Initial exposure to the virus can result in
severe respiratory disease, but subsequent exposures will only
result in mild to subclinical disease due to developed immunity.
Symptoms associated with the viral infection include high fever
(104-108°F), depression, decreased feed intake, increased
respiratory rate, cough, and nasal and lacrimal discharge.
Dyspnea, possibly with open-mouthed breathing, may occur in the
later stages of the disease. Complications include secondary
bacterial pneumonia. Lesions in the respiratory tract may occur.
BRSV is difficult to detect by culture, and
the chances of isolation only improve when sampling animals that
are in the incubation or acute phases of infection. Serological
detection may be used, but paired samples are required, and this
may take several weeks to accomplish. Molecular detection by PCR
can provide highly specific, rapid and sensitive detection of
Help confirm the disease causing agent
Identify BRSV carriers
Screen research materials for the presence of BRSV
Help ensure that animal groups are free of BRSV
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive from
Thonur, L., Maley, M., Gilray, J., Crook, T., Laming, E.,
Turnbull, D., Nath, M. and Willoughby, K. (2012)
multiplex real time RT-PCR for the detection of bovine
respiratory syncytial virus, bovine herpesvirus 1 and bovine
parainfluenza virus 3. BMC Vet. Res. 8:37-45
0.2 ml whole blood in EDTA (purple top) or ACD
(yellow top) tube, or 0.2 ml cell culture, or swab, or tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
for more information.
Turnaround time: 2 business days
Methodology: Qualitative reverse
transcription coupled real time PCR