We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

AMPKγ3R200Q mutation in pigs

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species


Aujeszky's disease

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Avibacterium paragallinarum

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea type 1

Brachyspira pilosicoli


Cache Valley virus




Caprine arthritis-encephalitis (CAE) virus

Chlamydia/Chlamydophila genus

Chlamydophila psittaci

Classical swine fever






Coxiella burnetii



Ebola Reston

E. coli O157:h7



Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowl adenovirus


Fusobacterium necrophorum

Hepatitis E

Herpes, avian


Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza type A

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Lawsonia intracellularis


Lumpy skin disease virus


Malignant catarrhal fever (MCF)


Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine hemorrhagic enteropathy

Porcine intestinal adenomatosis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel



Psittacine beak and feather disease

Psittacine herpes

Q fever



Rift Valley fever virus

Rinderpest virus

RyR1 R615C mutation in pigs


Staphylococcus xylosus

St. Louis encephalitis



Swine vesicular disease

Taenia solium

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichinella spiralis



Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Histoplasma PCR test for birds and livestock

avian & livestock assay data sheet


Test code:
F0006 - Ultrasensitive qualitative detection of Histoplasma species by real time polymerase chain reaction

Histoplasma is a genus of dimorphic fungi belonging to the family Ajellomycetaceae which are commonly found in bird and bat fecal material. The Histoplasma genus includes H. capsulatum which causes histoplasmosis; H. farciminosum which causes epizootic lymphangitis in horses; and H. duboisii which causes African histoplasmosis.

H. capsulatum is most prevalent in the Ohio and Mississippi river valleys. The disease caused by H. capsulatum is called histoplasmosis, also known as "cave disease," "Darling's disease," "Ohio Valley disease," "Reticuloendotheliosis," "spelunker’s lung" and “caver's disease.” Symptoms primarily affect the lungs, but other organs can be affected if the fungus spreads in the body. Histoplasmosis is commonly found in immunocompromised individuals such as AIDS patients and cancer patients.

People can be infected by inhaling microscopic fungi borne from bird or bat feces, or decomposing human biological fluids including urine, vomit, and feces.

Histoplasmosis can be diagnosed by detection of antigens in blood or urine samples by immunological or molecular methods. However, immunology is not very specific because antigens of Histoplasma can cross-react with antigens of blastomycosis, coccidioidomycosis, paracoccidioidomycosis and Penicillium marneffei infection. Formal histoplasmosis diagnoses may be confirmed by culturing the fungus directly. However, cultures may take up to 6 weeks for diagnostic growth to occur. Molecular detection by polymerase chain reaction (PCR) can provide a rapid, specific and sensitive method for diagnosis of this fungus (Elias et al., 2012).


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Histoplasma infection.
  • Help ensure that flocks and animal groups are free of this fungus
  • Early prevention of spread of this fungus
  • Minimize personnel exposure to this fungus
  • Safety monitoring of biological products and vaccines that derive from birds and susceptible livestock

Elías, N.A., Cuestas, M.L., Sandoval, M., Poblete, G., Lopez-Daneri, G., Jewtuchowicz, V., Iovannitti, C. and Mujica, M.T. (2012) Rapid identification of Histoplasma capsulatum directly from cultures by multiplex PCR. Mycopathologia. 174:451-456.

Specimen requirements: 0.2 ml bird or bat feces, or 0.2 ml whole blood in EDTA (purple top) tube, or urine, or sputum, or bronchoalveolar lavage, or fecal swab, or 0.2 ml fresh, frozen or fixed tissue, or environmental surface swab.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

©2003-2023 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880