We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

AMPKγ3R200Q mutation in pigs

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species


Aujeszky's disease

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Avibacterium paragallinarum

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea type 1

Brachyspira pilosicoli


Cache Valley virus




Caprine arthritis-encephalitis (CAE) virus

Chlamydia/Chlamydophila genus

Chlamydophila psittaci

Classical swine fever






Coxiella burnetii



Ebola Reston

E. coli O157:h7



Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowl adenovirus


Fusobacterium necrophorum

Hepatitis E

Herpes, avian


Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza type A

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Lawsonia intracellularis


Lumpy skin disease virus


Malignant catarrhal fever (MCF)


Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine hemorrhagic enteropathy

Porcine intestinal adenomatosis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel



Psittacine beak and feather disease

Psittacine herpes

Q fever



Rift Valley fever virus

Rinderpest virus

RyR1 R615C mutation in pigs


Staphylococcus xylosus

St. Louis encephalitis



Swine vesicular disease

Taenia solium

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichinella spiralis



Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Bovine herpesvirus type 2 PCR test
avian & livestock assay data sheet

Bovine herpesvirus type 2 (BoHV2)

Test code: S0216 - Ultrasensitive detection of bovine herpesvirus 2 by real time PCR

Bovine herpesvirus type 2 (BoHV-2) is also known as bovine mammillitis virus. It belongs to subfamily Alphaherpesvirinae, genus Simplexvirus.

Infection of cows with this virus may result in an acute, ulcerative condition of teat and udder skin referred to as bovine ulcerative mammillitis. This often results in reduced milk production and increased susceptibility to bacterial mastitis. However, not all infected cows show acute symptoms; infected animals may develop clinical symptoms ranging from relatively mild, small plaques of edema to severe ulceration. Early signs vary among infected animals, but the lesions often begin as one or more thickened, edematous plaques of varying size on the skin of one or more teats. Vesicles develop and may rapidly rupture, leaving a raw, ulcerated area that becomes covered with a dark-colored scab. The scabs tend to crack and bleed, especially if milking is attempted. Because the infection causes teats to be painful, infected cows often resist milking, leading to development of mastitis.

The greatest incidence is often seen in first-lactation cows, but previously unexposed cows of any age are susceptible. Severe lesions may take several weeks to heal. Infected animals may become latent carriers, and re-activation of the virus may occur.

Viral isolation has been used to diagnose this infection, but culture detection is not definitive. Serological detection requires time for infected animals to develop a detectable immune response. However, molecular detection by PCR is rapid, sensitive and specific, and is a good alternative to traditional methods (Campos et al., 2014).


  • Help confirm the disease causing agent
  • Identify BoHV2 virus carriers
  • Help ensure that herds are free of BoHV2
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

Campos, F.S., Franco, A.C., Oliveira, M.T., Firpo, R., Strelczuk, G., Fontoura, F.E., Kulmann, M.I., Maidana, S., Romera, S.A., Spilki, F.R., Silva, A.D., Hübner, S.O. and Roehe, P.M. (2014)  Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction. Vet. Microbiol. 171:182-188.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) tube, or lesion swab or scab, or 0.2 ml milk, or 0.2 ml fresh, frozen or fixed tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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