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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

AMPKγ3R200Q mutation in pigs

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species


Aujeszky's disease

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Avibacterium paragallinarum

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea type 1

Brachyspira pilosicoli


Cache Valley virus




Caprine arthritis-encephalitis (CAE) virus

Chlamydia/Chlamydophila genus

Chlamydophila psittaci

Classical swine fever






Coxiella burnetii



Ebola Reston

E. coli O157:h7



Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowl adenovirus


Fusobacterium necrophorum

Hepatitis E

Herpes, avian


Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza type A

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Lawsonia intracellularis


Lumpy skin disease virus


Malignant catarrhal fever (MCF)


Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine hemorrhagic enteropathy

Porcine intestinal adenomatosis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel



Psittacine beak and feather disease

Psittacine herpes

Q fever



Rift Valley fever virus

Rinderpest virus

RyR1 R615C mutation in pigs


Staphylococcus xylosus

St. Louis encephalitis



Swine vesicular disease

Taenia solium

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichinella spiralis



Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Infectious coryza PCR test for poultry
avian & livestock assay data sheet

Infectious coryza

Test code:
B0038 - Ultrasensitive qualitative detection of infectious coryza (Avibacterium paragallinarum bacteria; formerly Hemophilus paragallinarum) by real time polymerase chain reaction

B0038 is included in the poultry respiratory panel


Infectious coryza (IC) is an infectious respiratory disease found in many avian species worldwide. The causative agent of IC is Avibacterium paragallinarum (formerly known as Haemophilus paragallinarum). The disease can be acute to subacute at onset but typically progresses to a chronic state, with infected birds becoming carriers of the bacteria. Common names for the disease include croup, cold and coryza. In the United States, it is predominantly found in small noncommercial, menagerie, or hobby flocks. While chickens are the primary host of infectious coryza, pheasants, guinea fowl and turkeys are also susceptible.

Chronically ill or inapparent healthy carrier birds are the reservoir of infection. The source of the disease is often the addition of carrier birds to the flock. Recovered birds remain carriers and shedders for life. Transmission occurs by direct bird to bird contact, inhalation of infectious aerosols coughed into the air, or by ingestion of contaminated feed and water. The organism can be transferred on contaminated clothing, equipment and other fomites.

Coryza usually occurs in juvenile and mature birds 14 weeks of age or older. Incubation period is 1 to 3 days and the course of the acute disease stage is 4 to 12 weeks. Symptoms include nasal discharge, facial swelling, sneezing, labored breathing and fetid odor of the exudates. The infection mainly involves the upper respiratory tract--trachea, sinuses and air passages of the head.

A. paragallinarum bacteria have a polar staining and pleomorphic rod morphology. The bacterium is non-motile and gram negative. There are three antigenic types (A, B, and C) which all share certain antigens. H. paragallinarum requires both “X” and “V” factors in the media to grow.

Culture identification has traditionally been used to identify this bacterium. However, the stringent requirements for sample handling for culture for this bacterium, long incubation time and the occurrence of numerous variants of the bacteria have made culture difficult to perform and not optimally sensitive. Serological detection of A. paragallinarum is limited by availability of good monoclonal antibodies and the sensitivity of the individual assay protocol. Molecular detection by PCR has been confirmed to be the most sensitive, specific and rapid way of identifying this bacterium (Blackall, 1999).


  • Help confirm the disease causing agent
  • Environmental monitoring
  • Help ensure that bird populations are free of A. paragallinarum
  • Early prevention of spread of this bacterium among bird populations
  • Minimize human exposure to this bacterium
  • Safety monitoring of biological products and vaccines that derive from birds

Blackall, P.J. (1999) Infectious Coryza: Overview of the Disease and New Diagnostic Options. Clin Microbiol Rev. 12: 627–632.

Specimen requirements: Throat swab or sinus swab.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative PCR

Normal range: Nondetected

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