Moving reptiles?  Use our snake and lizard quarantine PCR panel to avoid spreading contagious agents.

Ruminating about hoofstock issues?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

Our Rodent Infestation PCR Panel tests for 5 common pathogens found in rodent-contaminated facilities.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Anisakis worms



Bacillus species

Batrachochytrium dendrobatidis

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Borna virus

Borrelia burgdorferi



Canine circovirus

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Canine parvovirus

Capillaria xenopodis


Chlamydophila pneumoniae

Chytrid fungus

Citrobacter freundii

Classical swine fever





Coxiella burnetii



Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel



Enterobacter cloacae


Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Francisella tularensis




Hepatitis E

Herring worms


Influenza type A

Influenza type B

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses


Lawsonia intracellularis




Listeria monocytogenes

Lizard quarantine panel

Lyme disease

Macropodid (kangaroo) herpesviruses


Mink enteritis virus


Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Ophidiomyces ophiodiicola

Pasteurella multocida

Pentastomid worms

Plasmodium species

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudocapillaroides xenopi

Pseudoloma neurophilia


Pseudoterranova worms

Q fever


Raillietiella orientalis


Reovirus screen


Rift Valley fever



Sarcocystis neurona

Snake fungal disease

Snake quarantine panel

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Tongue worms

Toxoplasma gondii

Treponema pallidum


Trypanosoma cruzi

Trypanosoma evansi


Turtle fraservirus


Valley Fever

Vesicular stomatitis


West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis

Toxoplasma gondii PCR test
wildlife and zoo assay data sheet

Toxoplasma gondii

Test code:
X0002 - Ultrasensitive qualitative detection of Toxoplasma gondii by real time polymerase chain reaction


Infections by Toxoplasma gondii are prevalent in many species of domestic and wild animals (Dubey, 1993). Although chronic infection of this obligate parasite is usually asymptomatic, T. gondii has emerged as a major opportunistic pathogen in the immunocompromised, such as AIDS patients. Surprisingly, spontaneous cases of fulminating fatal toxoplasmosis have been reported in adult squirrel monkeys (Saimiri sciureus) which are immunologically normal (Inoue, 1997). While the disease mechanism of this fatal toxoplasmosis in squirrel monkeys is not clear, infections of nonhuman primates with this parasite are common, and it has been shown that New World monkeys are more susceptible to T. gondii infection than Old World monkeys (Ruch, 1959). The outbreak of fatal toxoplasmosis (Cunningham, et al., 1992) is a major concern in caring for these primate colonies, as a recent study has indicated the possibility of horizontal transmission through the respiratory route (Furuta, et al., 2001) in addition to the fecal-oral route.

The “gold standard” for the detection of T. gondii in clinical specimens is mouse inoculation and detection of T. gondii specific antibodies. This method is sensitive and specific but very time-consuming, taking up to six weeks to obtain a diagnosis. Cell culture detection of this parasite is also slow, and lacks sensitivity. PCR detection of this parasite has been found to be a sensitive, specific and rapid method for the detection of T. gondii DNA in a wide spectrum of samples, such as amniotic fluid (Grover, et al., 1990), blood (Dupouy-Camet, et al., 1993; Ho-Yen, et al., 1992), tissue samples (Johnson, et al., 1993) and cerebrospinal fluid (Cristina, et al., 1993; Farmley, et al., 1992). Although serology testing can help diagnose recent infection with this parasite, PCR testing is found to be more sensitive in identifying acute infection (Hussein, et al., 2002).


  • Help confirm the disease causing agent
  • Help ensure that animal groups and populations are free of T. gondii
  • Early prevention of spread of this parasite among a population
  • Minimize human exposure to this parasite

Dubey, J.P. (1993) Toxoplasma, Neoplasma, Sarcocystis, and other tissue cyst-forming coccidian of human and animals. pp1-56. In: Parasitic protozoa (Kreier, P.J. ed), vol. 6, 2nd ed., Academic Press, Inc., San Diego, California.
Inoue, M. (1997) Acute toxoplasmosis in squirrel monkeys. J. Vet. Med. Sci. 59:593-595.
Ruch, T.C. (1959). pp.297-299, 313-318, 423-424. In: Diseases of laboratory primates. W.B. Saunders Co., Philadelphia.
Cunningham, A.A., Buxton, D. and Thomson, K.M. (1992) An epidemic of toxoplasmosis in a captive colony of squirrel monkeys (Saimiri sciureus). J. Comp. Pathol. 107:207-219.
Furuta, T., Une, Y., Omura, M., Matsutani, N., Nomura, Y., Kikuchi, T., Hattori, S. and Yoshikawa, Y. (2001) Horizontal transmission of Toxoplasma gondii in squirrel monkeys (Saimiri sciureus). Exp. Anim. 50:299-306.
Grover, M.C., Thulliez, P., Remington, J.S. and Boothroyd, J.C. (1990) Rapid prenatal diagnosis of congenital Toxoplasma infection by using polymerase chain reaction and amniotic fluid. J. Clin. Microbiol. 28:2297-2301.
Dupouy-Camet, J., de Souza, S.L., Maslo, C., Paugam, A., Saimot, A.G., Benarous, R., Tourte-Schaefer, C. and Derouin, F. (1993) Detection of Toxoplasma gondii in venous blood from AIDS patients by polymerase chain reaction. J. Clin. Microbiol. 31:1866-1869.
Ho-Yen, D.O., Joss, A.W.L., Balflour, A.H., Smyth, E.T.M., Baird, D. and Chatterton, J.M.W. (1992) Use of the polymerase chain reaction to detect Toxoplasma gondii in human blood samples. J. Clin. Pathol. 45:910-913.
Johnson, J.D., Butcher, P.D., Savva, D. and Holliman, R.E. (1993) Application of the polymerase chain reaction to the diagnosis of human toxoplasmosis. J. Infect. 26:147-158.
Cristina, N.H., Pelloux, C., Goulhot, J.P., Brion, P., Leclercq, P. and Ambrosis-Thomas, P. (1993) Detection of Toxoplasma gondii in AIDS patients by the polymerase chain reaction. Infection 21:150-153.
Farmley, S.F., Goebel, F.D. and Remington, J.S. (1992) Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by polymerase chain reaction. J. Clin. Microbiol. 30:3000-3002.
Hussein, A.H., Nagaty, I.M. and Fouad, M.A. (2002) Evaluation of IgM-ELISA versus PCR in diagnosis of recent Toxoplasma gondii infection. J Egypt Soc Parasitol. 32:639-46.

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml feces, or 0.2 ml amniotic fluid or CSF, or 0.2 ml fresh, frozen or fixed tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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