environmental assay data sheet
Bacillus PCR test
code: B0113 -
Ultrasensitive qualitative detection of Bacillus
bacteria by real time PCR
Bacillus is a genus of
rod-shaped gram-positive bacteria that consists of at least 266
species. These bacteria are either obligate aerobes or
facultative anaerobes, and are usually catalase positive. One
major characteristic of these bacteria is the formation of
spores which allow these bacteria to remain dormant for years.
These bacteria occur ubiquitously. Some of the
Bacillus bacteria can
cause significant human diseases. For example,
B. anthracis can cause
anthrax in human; ingestion of
B. cereus leads to
severe food poisoning; B.
subtilis infection can lead to endocarditis, pneumonia, and
septicemia especially in patients with immunological disorders.
Because of the ubiquitous nature of these bacteria,
contamination of medical instruments and food products can be of
significant concern, especially to people with compromised
Bacillus bacteria can also be
useful to humans. For example,
B. subtilis produces
amylase which is widely used in industrial and agricultural
processing applications due to its high degree of stability.
B. thuringiensis can
produce Cry proteins which are natural pesticides.
Culture detection of these bacteria has been used to provide a
presumptive diagnosis of infection. However, due to many
bacteria sharing the same phenotypic and biochemical
characteristics as Bacillus bacteria, confirmation of infection requires exhaustive
testing. Molecular detection by polymerase chain reaction is an
alternative which provides highly sensitive and specific
confirmation of the presence of these bacteria.
Bacillus species detected by
this assay include B.
subtilis, B. anthracis, B. cereus, B. tequilensis, B.
vallismortis, B. inaquosorum, B. intestinalis, B.
stratosphericus, B. spizizenii, B. amyloliquefaciens, B.
inaquosus, B. tequilensis, B. vallismortis, B. mojavensis, B.
licheniformis, B. vallismortis, B. flexus, B. velezensis, B.
halotolerans, B. thuringiensis, B. tropicus, B.
paralicheniformis, B. paranthracis, B. wiedmannii, B. pacificus,
B. toyonensis, B. mycoides, B. cabrialesii, B. rugosus, B.
haikouensis, B. nitratireducens. B. siamensis, B. haynesii.
Check for Bacillus species in facilities, buildings, soil, ventilation systems, or goods
Selection of appropriate remediation regimens
Check for post-remediation absence of Bacillus
Help confirm the presence of disease-causing agents in
Early prevention of the spread of Bacillus
species among animals in facilities
Help minimize human exposure to Bacillus
Priha, O. et al. (2004) Detection of Bacillus cereus group bacteria from
cardboard and paper with real-time PCR. J. Industrial Microbiol.
and Biotechnol., 31: 161–169
Specimen requirements: 0.2 ml cell culture, or environmental surface swab (cotton or synthetic tip) or swipe
pad (approx 2" x 2"), or 2 ml of soil, or 0.2 ml feces
or fecal swab, or 0.2 ml EDTA whole blood, or 0.2 ml fresh or
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time polymerase chain reaction