wildlife and zoo assay data sheet
Test code: B0087 - Qualitative ultrasensitive detection of
by real time PCR.
Delftia acidovorans was formerly called
Comamonas acidovorans or Pseudomonas acidovorans.
Prior to 1987, a number of now-independent genera, including
Delftia, Burkholderia, Stenotrophomonas, Ralstonia, Comamonas,
Acidovorax and others, were all grouped within the broad
genus Pseudomonas. In general, these bacteria are
aerobic, oxidase-positive, Gram-negative bacilli that grow on
MacConkey's agar. When this broad genus was rearranged based on
rRNA sequencing data, D. acidovorans was grouped into
the family Comamonadaceae based on 16S rRNA gene sequence
analysis. However, Comamonas acidovorans was later
found to be phylogenetically quite distant from the other
species of Comamonas (Wen et al., 1999), and was
therefore renamed Delftia acidovorans.
Morphologically, D. acidovorans
appears as straight to slightly curved Gram-negative bacilli,
occurring singly or in pairs. Strains are motile by means of
polar or bipolar tufts of one to six flagella. The organism is
strictly aerobic and is a common water and soil saprophyte.
D. acidovorans is normally of limited virulence and is
usually considered an opportunistic pathogen. Due to its
widespread occurrence, it is often implicated in the
contamination of medical devices such as intravascular catheters
Culture differentiation of D.
acidovorans can be difficult because it shares many
biochemical and growth characteristics with other bacteria in
the Pseudomonas genus. Molecular detection by PCR is
highly specific and sensitive and can often be used instead of
culture to detect and identify D. acidovorans
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of Delftia acidovorans infection
Help ensure animal groups and populations are free of
Help ensure environmental surfaces and devices are free of
Early prevention of spread of these bacteria
among a population
Minimize human exposure to
Safety monitoring of biological products and vaccines
that derive from susceptible animals
Wen , A.,
Fegan, M., Hayward, C., Chakraborty, S. and Sly, L.I. (1999)
Phylogenetic relationships among members of the Comamonadaceae,
and description of Delftia acidovorans (den Dooren de
Jong 1926 and Tamaoka et al. 1987) gen. nov., comb. nov. Int. J.
Syst. Bacteriol. 49:567-76.
ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml urine, or
swab, or nasopharyngeal swab, or rectal swab, or 0.2 ml feces.
if advice is needed to determine an appropriate specimen type
for a specific diagnostic application. For specimen
types not listed here, please contact Zoologix to confirm
specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time PCR