Leptospira PCR test
wildlife and zoo assay data sheet
B0051 - Ultrasensitive qualitative detection of
by real time polymerase chain reaction. This test detects but
does not differentiate common
Leptospirosis is a disease of worldwide significance that
infects many domestic and wildlife animal species and humans.
The scientific name of the infecting organism is
Leptospira interrogans sensu
lato. Within this species there are many different
can be spread between animals through contact with infected
urine, venereal or placental transfer, or bite wounds. Indirect
transmission occurs through exposure of susceptible animals to
contaminated water sources, food, bedding or human hands.
can survive in feces up to 2 months. Stagnant or slow moving
water provides a suitable habitat; the organism can survive up
to 20 days in water. This explains why outbreaks often increase
during periods of flooding.
shivering and muscle tenderness are among the first signs of
acute infection. Rapid dehydration may develop subsequently. In
subacute infections, the animal usually develops a fever,
anorexia, dehydration, and increased thirst. Animals with liver
involvement may develop icterus. Conjunctivitis and recurrent
uveitis may occur in chronic infections. Breeding success can be
impacted because of increased risk of abortion and decreased
can be diagnosed by change in antibody titers, titers may be
negative during early stages of critical infection. Thus an
alternative method is needed to provide a quick diagnosis of the
disease at this stage. Molecular detection of PCR is rapid,
highly sensitive and specific. It is suitable for quick
diagnosis even during the first few days of infection.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of Leptospira
Help ensure that animal populations are free of
Early prevention of spread of this bacterium
Minimize personnel exposure to this bacterium
Safety monitoring of biological products that derive
from susceptible animals
swab, or cervical swab, or 0.2 ml urine, or 0.2 ml fresh, frozen
or fixed tissue.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
real time PCR