wildlife, zoo and aquatic assay data sheet
- Ultrasensitive qualitative detection of Edwardsiella
bacteria by real time PCR.
This assay detects but does not differentiate
Edwardsiella is a genus of gram-negative,
facultatively anaerobic, rod-shaped bacteria of the family
Enterobacteraceae. They are considered occasional opportunistic
pathogens of humans. There are three species in the genus:
1. Edwardsiella hoshinae has been isolated from animals and humans. It does not produce indole but
is motile. E. hoshinae
infects birds and reptiles.
2. Edwardsiella ictaluri is nonmotile and does not produce indole. It usually occurs as a pathogen
3. Edwardsiella tarda is also known as
Edwardsiella anguillimortifera. It produces indole, and is
biochemically very similar to
Escherichia coli. It
is usually found in the intestinal tracts of aquatic animals and
reptiles. It can also occasionally be isolated from the urine,
blood, and feces of humans and has been known to cause
gastroenteritis and wound infections.
is the etiologic agent of edwardsiellosis, a devastating fish
disease that affects worldwide aquaculture industries.
These bacteria can be cultured with routine media but
identification requires special selective media. PCR enables
rapid and highly sensitive detection of these bacteria (Bilodeau
et al., 2003).
Help confirm the disease causing agent
Shorten the time required to confirm a clinical diagnosis of
Help ensure that animal facilities and populations are free of
Early prevention of spread of these bacteria
Minimize human exposure to these bacteria
Bilodeau, A. L., Waldbieser, G.C., Terhune, J.S., Wise,
D.J. and & Wolters, W.R. (2003) A Real-Time Polymerase Chain
Reaction Assay of the Bacterium Edwardsiella ictaluri in
Channel Catfish. J. Aquatic Animal Health 15: 80-86.
0.2 ml of whole blood in EDTA (purple top) or ACD (yellow top)
tube, or wound swab, or 0.2 ml feces or urine, or fecal swab, or
water sample, or bacterial culture, or food sample
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
Turnaround time: 2 business days
Qualitative real time PCR
Normal range: Nondetected