wildlife and zoo assay data sheet
- Ultrasensitive qualitative detection of Streptococcus pyogenes by
real time polymerase chain reaction.
is a gram-positive spherical-shaped bacterium. It is
beta-hemolytic and belongs to Lancefield serogroup A, also known
as group A streptococci (GAS).
The bacteria can cause a wide variety of diseases in
humans such as acute pharyngitis, accounting for 15%-30% of
cases in children and 5%-10% of cases in adults.
During the winter and spring in temperate climates, up to 20% of
asymptomatic school-aged children may be GAS carriers.
Although GAS usually causes pharyngitis or impetigo, it can
infrequently cause invasive diseases such as cellulitis,
bacteremia, necrotizing fasciitis and toxic shock syndrome
(TSS). Along with Staphylococcus aureus, GAS is one of the most
common pathogens responsible for cellulitis.
GAS is ubiquitous in nature and many animal species are carriers
of these bacteria. Close contact between
and humans can spread this infection in both directions.
Although culture may be used to detect these bacteria, molecular
detection by PCR is a rapid, highly sensitive and specific
method (Bexiga et al., 2011).
Help confirm the disease causing agent
Shorten the time required to confirm a clinical diagnosis of
S. pyogenes infection.
Help ensure that colonies are free of S. pyogenes
Early prevention of spread of this bacterium
Minimize personnel exposure to this bacterium
Safety monitoring of biological products that derive from
Specimen requirements: 0.2
ml whole blood in EDTA (purple top) tube, or serum, or bacterial culture; or nasal, pharyngeal or
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
2 business days
Qualitative real time PCR