Moving reptiles?  Use our snake and lizard quarantine PCR panel to avoid spreading contagious agents.

Ruminating about hoofstock issues?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

Our Rodent Infestation PCR Panel tests for 5 common pathogens found in rodent-contaminated facilities.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Anisakis worms

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Camelpox

Campylobacter

Canine circovirus

Canine distemper

Canine parvovirus

Capillaria xenopodis

Chlamydia/
Chlamydophila

Chlamydophila pneumoniae

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptococcosis

Cryptosporidium

Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Herring worms

Histoplasma

Influenza

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leishmania

Leptospira

Listeria monocytogenes

Lizard quarantine panel

Lyme disease

Macropodid (kangaroo) herpesviruses

Malaria

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Ophidiomyces ophiodiicola

Pasteurella multocida

Plasmodium species

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudocapillaroides xenopi

Pseudoloma neurophilia

Pseudorabies

Pseudoterranova worms

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Snake fungal disease

Snake quarantine panel

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Cryptococcosis PCR test

environmental/diagnostic assay data sheet

Cryptococcus neoformans

Test code:
F0003 - Ultrasensitive qualitative detection of Cryptococcus neoformans by real time polymerase chain reaction

 

Cryptococcus neoformans is a fungus that is found worldwide and is the etiologic agent of cryptococcosis. It is commonly associated with old pigeon manure, but it has also been recovered from dried excreta of chickens, sparrows, starlings, and other birds. C. neoformans uses the creatinine in avian feces as a nitrogen source. It thereby gains a competitive advantage over other microorganisms and multiplies exceedingly well in dry bird manure accumulated in places that are not in direct sunlight.

It is generally accepted that the organism enters hosts, including humans, by the respiratory route in the form of a dehydrated yeast or as spores. Most cryptococcosis infections are mild and occur without symptoms. Diffuse pulmonary infection is often asymptomatic and unrecognized. Persons with weakened immune systems due to AIDS, cancer or transplantation, however, are more susceptible to symptomatic infection.

The generalized form of cryptococcosis begins with a lung infection and spreads to other areas of the body, particularly the central nervous system, and is usually fatal if left untreated. The cutaneous form is characterized by acne-like skin eruptions or ulcers with nodules just under the skin. The cutaneous form is generally very rare in normal individuals.

Because contact with pet birds and their excreta can pose a significant health risk to immunocompromised patients, it is important that birds be screened for this fungus before being adopted. Culture identification is labor intensive and cultures are frequently contaminated by other fungi. Molecular detection by PCR provides quick, sensitive and specific detection of Cryptococcus neoformans in a variety of specimen types such as excreta and cloacal swabs.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Cryptococcus infection.
  • Help ensure that flocks are free of this fungus
  • Early prevention of spread of this fungus
  • Minimize personnel exposure to this fungus
  • Safety monitoring of biological products and vaccines that derive from birds

Specimen requirements: Cloacal swab, or 0.2 ml feces, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml CSF, or 0.2 ml fresh, frozen or fixed tissue.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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