Moving reptiles?  Use our snake and lizard quarantine PCR panel to avoid spreading contagious agents.

Ruminating about hoofstock issues?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

Our Rodent Infestation PCR Panel tests for 5 common pathogens found in rodent-contaminated facilities.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Anisakis worms

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Camelpox

Campylobacter

Canine circovirus

Canine distemper

Canine parvovirus

Capillaria xenopodis

Chlamydia/
Chlamydophila

Chlamydophila pneumoniae

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptococcosis

Cryptosporidium

Cryptosporidium serpentis

Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacter cloacae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Herring worms

Histoplasma

Influenza type A

Influenza type B

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leishmania

Leptospira

Listeria monocytogenes

Lizard quarantine panel

Lyme disease

Macropodid (kangaroo) herpesviruses

Malaria

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Ophidiomyces ophiodiicola

Pasteurella multocida

Plasmodium species

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudocapillaroides xenopi

Pseudoloma neurophilia

Pseudorabies

Pseudoterranova worms

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Snake fungal disease

Snake quarantine panel

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Influenza B PCR test
wildlife and zoo assay data sheet

Influenza type B

Test code: S0239 - Ultrasensitive qualitative detection of influenza B virus by reverse transcription coupled real time polymerase chain reaction. This assay does not detect influenza types A, C or D.

There are four types of influenza viruses: A, B, C and D. All influenza viruses are RNA viruses. Human influenza A and B viruses cause seasonal epidemics of disease. Influenza A viruses are the only influenza viruses known to cause flu pandemics, i.e., global epidemics of flu disease. Influenza A viruses have a very broad host range; they are also known to infect birds, pigs, horses, dogs and non-human primates.

Influenza B virus is only known to infect humans, seals and ferrets. Influenza B mutates at a rate two to three times slower than type A and consequently influenza B is less genetically diverse. Due to this lack of antigenic diversity, immunity to influenza B virus is usually acquired at an early age, though such immunity typically does not last a lifetime. Pandemics of influenza B infection are unlikely due to influenza B’s lower rate of antigenic change and its limited host range.

Influenza type C infections generally cause mild illness and are not thought to cause human flu epidemics. Influenza D viruses primarily affect cattle and are not known to infect or cause illness in people.

Influenza B virus is the only species in the genus Betainfluenzavirus of the family Orthomyxoviridae. The Influenza B viral genome consists of eight segments of linear negative-sense single-stranded RNA. Each RNA segment is enclosed in separate nucleocapsid, and the nucleocapsids are surrounded by one envelope. Influenza B viruses are not divided into subtypes but there are two recognized lineages of influenza B, B/Yamagata and B/Victoria.

Viral culture to detect influenza B virus is slow and not very sensitive. Antigen detection method is rapid but is not very sensitive. The current method of choice for detection of influenza B virus is molecular detection by polymerase chain reaction, which is highly sensitive and specific (Azar and Landry, 2018).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of influenza B
  • Help ensure that animal groups are free of influenza B
  • Early prevention of spread of this virus
  • Minimize personnel exposure to this virus

References:
Azar, M. M., & Landry, M. L. (2018). Detection of Influenza A and B Viruses and Respiratory Syncytial Virus by Use of Clinical Laboratory Improvement Amendments of 1988 (CLIA)-Waived Point-of-Care Assays: a Paradigm Shift to Molecular Tests. J. Clin. Microbiol. 56: e00367-18.

Specimen requirements:

Preferred sample - nasopharyngeal swab.

Less preferred sample - 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions. For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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