Hantavirus PCR test
environmental/diagnostic assay data sheet
Hantavirus PCR test
S0135 - Ultrasensitive qualitative detection of Hantavirus by
reverse transcription coupled real time PCR. This test does not
detect Seoul virus - see test code
S0224 for Seoul virus detection.
are a group of viruses that are transmitted by rodents through
urine, droppings, and saliva. One of them, Sin Nombre
virus, is found in deer mice in North America. Sin Nombre
virus is the cause of Hantavirus Pulmonary Syndrome (HPS) in
Hantaviruses belong to the Bunyaviridae family of
viruses. There are five genera in this family:
Orthobunyavirus, Nairovirus, Phlebovirus,
Hantavirus. Like all
members of this family, hantaviruses have a genome comprised of
three negative-sense single-stranded RNA segments, and so are
classified as negative sense RNA viruses.
of dust contaminated by rodent excreta or saliva can result in
transmission of these viruses to humans. Infected people will
develop HPS one to six weeks after inhaling the virus. The
disease begins with 2-6 days of flu-like illness including
fever, muscle soreness, headache, nausea, vomiting and fatigue.
As the disease progresses it causes shortness of breath due to
fluid filled lungs; hospital care is then required. It is
usually a serious infection and about 1 out of 3 infected
patients have died.
mouse (Peromyscus maniculatus) is the main carrier of
hantaviruses in the western United States but other wild rodents
may also be potential carriers. The deer mouse can carry and
shed the virus without showing any clinical signs. In one study,
it was shown that about 14% of over 1,100 deer mice in
Washington State had been infected with Sin Nombre virus.
HPS is not
known to be transmitted from one person to another, nor to be
transmitted by farm animals, dogs, cats or rodents purchased
from pet stores.
are presented in a concise, signed, easy-to-read PDF report
optimized for documentation and recordkeeping.
Detect this virus in surface dust, soil, water or other environmental
Confirm the presence of the disease causing agent in biological samples
Minimize occupational exposure to this virus
Specimen requirements: 1 rodent fecal pellet,
or a surface dust swab or
gauze pad, or 10 ml soil, or 10 ml water
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time polymerase chain reaction