wildlife and zoo assay data sheet
Ultrasensitive qualitative detection of
Stenotrophomonas maltophilia by real time PCR.
Stenotrophomonas maltophilia is
an aerobic, nonfermentative, gram-negative bacterium. Rare cases
of human infections have been described, and are difficult to
treat. The bacterium was initially classified as
but subsequently was re-grouped into the genus
Xanthomas. In 1993
it was again reclassified into the genus
S. maltophilia is
ubiquitous in aqueous environments, soil and plants, and
frequently colonizes respiratory and urinary tracts without
adverse effects. In immunocompetent individuals, symptomatic
infection with S. maltophilia is uncommon. However,
nosocomial infections are possible in immunocompromised
individuals. Contamination of biological products with these
bacteria during manufacturing processes is also a significant
Treatment of S. maltophilia-infected patients is
difficult because this bacterium is resistant to many
broad-spectrum antibiotics (including all carbapenems) due to
the production of two inducible chromosomal metallo-β-lactamases
(designated L1 and L2). Currently many strains of S. maltophilia
are still sensitive to co-trimoxazole and ticarcillin, but
resistant strains are being reported with increasing frequency.
Laboratory diagnosis of S. maltophilia infection traditionally
depended on culture identification, requiring a long incubation
time. However, molecular detection by polymerase chain reaction
(PCR) is a rapid, sensitive and specific method to detect these
bacteria (Cattoir et al., 2010).
Confirm the disease causing agent
Shorten the time required to confirm a clinical
Help ensure that facilities are free of this bacterium
Early prevention of spread of
S. maltophilia in
Minimize human exposure to this bacterium
Safety monitoring of biological products
Gilibert, A., Le Glaunec, J.M., Launay, N., Bait-Mérabet, L. and
Legrand, P. (2010) Rapid detection of Pseudomonas aeruginosa
from positive blood cultures by quantitative PCR. Ann. Clin.
Microbiol. Antimicrob. 9:21-25.
ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or nasopharyngeal swab, or 0.2 ml urine, or 0.2 ml
feces, or fecal swab, or 0.2 ml fresh, frozen or fixed tissue,
or environmental swab.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
Qualitative real time PCR