Yersinia pseudotuberculosis PCR test
dog and cat assay data sheet
Ultrasensitive qualitative detection of
by real time PCR.
Yersinia pseudotuberculosis is a gram-negative bacterium which primarily causes disease
in animals, although humans may also be infected through food
contamination. Infection with this pathogen
can cause tuberculosis-like symptoms,
including localized tissue necrosis and granulomas in the spleen,
liver, and lymph node.
The bacteria occur widely in various host species, including dogs,
cats, horses, cattle, rabbits, deer, rodents, and birds. Documented
cases of occupational exposure to
Y. pseudotuberculosis include butchers working in abattoirs
slaughtering swine. Outbreaks of
Y. pseudotuberculosis in animal facilities have occasionally been
reported, including in primate facilities (Bronson et al., 1972; Iwata
et al., 2008).
Successfully culturing Y.
pseudotuberculosis is very difficult, and serologic tests in many
cases require serial testing on multiple serum samples taken at
several time points. Molecular detection by PCR offers a rapid,
sensitive and specific method for detecting
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of Yersinia
Help ensure that animal facilities are free of
Early prevention of spread of
among a facility
Minimize human exposure to
Safety monitoring of biological products that derive
from susceptible animals
R.T., May, B.D. and Ruebner, B.H. (1972) An Outbreak of Infection by
Yersinia pseudotuberculosis in Nonhuman Primates. Am. J. Pathol. 69:
Iwata, T., Une, Y., Okatani, A.T., Kato, Y., Nakadai, A.,
Lee, K., Watanabe, M., Taniguchi, T., Elhelaly, A.E., Hirota, Y. and
Hayashidani, H. (2008) Virulence characteristics of Yersinia
pseudotuberculosis isolated from breeding monkeys in Japan. Vet
Rectal swab, or 0.2 ml feces, or 0.2 ml fresh, frozen or fixed tissue,
or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all specimen
types, if there will be a delay in shipping, or during very warm
weather, refrigerate specimens until shipped and ship with a cold pack
unless more stringent shipping requirements are specified. Frozen
specimens should be shipped so as to remain frozen in transit. See
shipping instructions for more
2 business days
polymerase chain reaction