NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Fading kitten syndrome

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Group G strep

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus, Group G

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Chikungunya PCR test

dog and cat assay data sheet

Chikungunya virus

Test code:
S0203 - Ultrasensitive qualitative detection of chikungunya virus by reverse transcription coupled real time polymerase chain reaction

Chikungunya virus is transmitted by mosquito. The most common symptoms of chikungunya infection are fever and joint pain, which can be excruciating, leading to the colloquial name “breakbone fever.” Other symptoms may include headache, muscle pain, joint swelling, and rash. Chikungunya means "that which bends up" or "to be contorted" in the Kimakonde language and was first described in the 1950s in Tanzania. Chikungunya is an RNA virus in the Alphavirus genus of the Togaviridae family.

In 2013, chikungunya viral infection was reported in humans in the Caribbean. The virus quickly spread to almost every island, with many cases found in the Dominican Republic and Haiti. In 2014, cases of this viral infection started to be reported in Central and South America and it quickly became endemic. As of September 5, 2014, the Pan American Health Organization had confirmed 8210 cases and 37 deaths resulting from chikungunya viral infection, with 751 reported cases in the continental United States, mostly in southern Florida.

Two species of day-time biting mosquito transmit chikungunya virus:  Aedes aegypti, which only bites humans; and Aedes albopictus (the Asian "tiger mosquito"), which has become common in much of the Caribbean and southesastern United States and bites not just people but a range of animals, including squirrels, deer, birds and dogs.

Diagnosis of chikungunya viral infection is mostly based on serological and PCR techniques. Viral culture followed by viral antigen detection is a sensitive method but must be performed under BSL3 biosafety conditions, and is time consuming and labor intensive, limiting its usefulness. Serological methods are reliable but are not appropriate in early stage infection, i.e., before 5–6 days after clinical onset, because antibody titer is low during early stages of infection and serology is not sensitive enough to detect small changes in antibody titer. Nucleic acid amplification by PCR is an appropriate diagnostic tool at an early stage of infection, while the patient is viremic (Laurent et al., 2007).

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that pets and kennels are free of this virus
  • Early prevention of spread of this virus among a kennel
  • Minimize human exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Apandi, Y., Nazni, W.A., Noor Azleen, Z.A., Vythilingam, I., Noorazian, M.Y. , Azahari, A.H., Zainah, S. and Lee, H.L. (2009) The first isolation of chikungunya virus from nonhuman primates in Malaysia. J. Gen. Mol. Virol. 1: 035–039

Laurent, P., Le Roux, K., Grivard, P., Bertil, G., Naze, F., Picard, M., Staikowsky, F., Barau, G., Schuffenecker, I. and Michault, A. (2007) Development of a sensitive real-time reverse transcriptase PCR assay with an internal control to detect and quantify Chikungunya virus. Clin. Chem. 53: 1408–1414.

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml plasma or serum, or 0.2 ml cerebrospinal fluid, or 0.2 ml tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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