NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Fading kitten syndrome

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Group G strep

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus, Group G

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Anaplasma phagocytophilum PCR test for dogs and cats

 dog and cat assay data sheet

Anaplasma phagocytophilum

Test code:
B0101 - Ultrasensitive detection of Anaplasma phagocytophilum (formerly Ehrlichia phagocytophilum) by real time PCR

 

Anaplasma phagocytophilum is a gram-negative bacterium formerly known as Ehrlichia phagocytophilum. It causes anaplasmosis in sheep and cattle, also known as tick-borne fever and pasture fever respectively. The bacteria tend to attack neutrophils and when they infect humans, they can cause human granulocytic anaplasmosis.

The bacteria are transmitted to humans by the bite of an infected tick. The black-legged tick (Ixodes scapularis) is the vector of A. phagocytophilum in the northeastern and upper midwestern United States. The western black-legged tick (Ixodes pacificus) is the primary vector in Northern California.

When humans are infected, it may take one to two weeks before symptoms develop. Symptoms include fever, headache, muscle pain, skin rash (in rare occasions), leukopenia, thrombocytopenia and mild injury to the liver.

When animals are infected with these bacteria, they may develop lethargy, ataxia, inappetence, and weak or painful limbs, but the most severe changes are anemia and leukopenia. The infection is often confused with Lyme disease, another tick-borne illness.

Serological detection of these bacteria can take some time to reach a definitive diagnosis. Molecular detection by polymerase chain reaction (PCR) is a highly specific and sensitive way to detect the presence of these bacteria, and should be considered as an alternative to the serological method (Kirtz et al., 2005).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Anaplasma infection
  • Help ensure that herds are free of Anaplasma
  • Early prevention of spread of Anaplasma among animals
  • Minimize human exposure to Anaplasma
  • Safety monitoring of biological products that derive from susceptible animals.

References:
Kirtz, G., Meli, M., Leidinger, E., Ludwig, P., Thum, D., Czettel, B., Kölbl, S. and Lutz, H. (2005) Anaplasma phagocytophilum infection in a dog: identifying the causative agent using PCR. J. Small Anim. Pract. 46:300-303.

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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