NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline syncytial virus

Francisella tularensis

Giardia

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Pasteurella multocida PCR test for dogs and cats

dog and cat assay data sheet

Pasteurella multocida

Test code: B0045 - Ultrasensitive qualitative detection of Pasteurella multocida by real time PCR

 

Pasteurella bacteria are small, nonmotile, gram-negative, bi-polar-staining bacilli that can be easily identified in many dog and cat bite wounds. These organisms normally inhabit the nasal, gingival, and tonsillar regions of many dogs and most cats, as well as those of many other animals.

Pasteurella secretes an endotoxin that changes the properties of pulmonary surfactant, thereby altering pulmonary mechanics and gas exchange. Once pneumonia develops, it may be slow to resolve and abscesses or pleuritis may develop.

Pasteurella multocida is the most common respiratory pathogen in the domestic rabbit. Infection with the bacteria can result in rhinitis, conjunctivitis, pneumonia, abscesses, genital tract infections, and septicemia. The bacteria can be transmitted via aerosol or contact, either directly or through fomites, with an infected rabbit. P. multocida initially colonizes the pharynx and then moves to the nasal cavity and surrounding tissue, with possible spread to the rest of the body. Colonization of the nasal cavity may take two weeks to occur, after which time clinical signs may or may not appear. Once rabbits are infected, they become carriers without exhibiting clinical signs.

Traditionally, diagnosis of Pasteurella was based on clinical findings, culture and/ or serological testing. Although culture identification methods are definitive, they are time consuming and costly. False negative culture results are frequently observed due to the fact that P. multocida dies easily during transport to the laboratory or is overgrown by other bacteria (nasal flora or contaminants) in the culture. Serology has been used when infection is suspected in organs for which cultures are not attainable, or when culturing has yielded no results. However, a serology positive to P. multocida can indicate either current infection or previous exposure to the organism. Because many animals have been exposed to this organism, a current diagnosis of pasteurellosis cannot be based on serologic results alone. Molecular detection by PCR offers a more sensitive, specific and timely method for detecting Pasteurella and confirming infection (Miflin and Blackall, 2001).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Pasteurella
  • Help ensure that animal facilities are free of Pasteurella
  • Early prevention of spread of Pasteurella among a facility
  • Minimize personnel exposure to Pasteurella
  • Safety monitoring of biological products that derive from susceptible animals

References:
Miflin, J. K. and Blackall, P. J. (2001) Development of a 23 S rRNA-based PCR assay for the identification of Pasteurella multocida. Lett. Appl. Microbiol. 33: 216–221.

Specimen requirements: Nasopharyngeal swab, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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