Top dogs can catch things too!  Our NEW dog show panel checks for 8 pathogens potentially transmissible at dog shows.

 Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 8 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella canis

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

Demodex gatoi mites

E. coli

Ehrlichia

Fading kitten syndrome

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Group G strep

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

Mange in cats

Microsporum

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neorickettsia helmintheca

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Ringworm

Salmonella

Salmon poisoning disease

Sarcocystis neurona

Streptococcus, Group G

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trichophyton

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Brucella canis PCR test

dog and cat assay data sheet

Brucella canis

Test code: B0110 - Ultrasensitive detection of Brucella canis bacteria by real time PCR. This assay does not detect Brucella abortus, B. microti, B. melitensis, B. pinnipedialis, B. suis, B. ovis or B. neotomae.

B0110 is included on P0034 - canine breeding panel and P0051 - dog show panel

Canine brucellosis is an important cause of reproductive failure, particularly in kennels. The disease, which is caused by the bacteria Brucella canis, can result in abortions, stillbirths, epididymitis, orchitis and sperm abnormalities in dogs. Other Brucella species, such as B. abortus, B. melitensis, and B. suis, can also cause brucellosis in dogs but the occurrence of those species in dogs is very rare. B. canis can also infect humans but the virulence is low, and only a few cases with mild symptoms have been documented.

B. canis can be detected in the fetus, placenta, fetal fluids and vaginal discharge after an abortion or stillbirth caused by the bacteria. The bacteria will persist in vaginal discharges for several weeks after an abortion, and will be shed in normal vaginal secretions, particularly during estrus, as well as in milk. High concentration of B. canis may be found in semen for weeks or months after infection, and intermittent shedding of smaller quantities can occur for years. Infected dogs may shed the bacteria in urine, and low concentrations of bacteria may also be shed in saliva, nasal and ocular discharge, and feces.

Although B. canis mainly enters the body by ingestion and via the genital, oronasal and conjunctival mucosa, transmission through broken skin has been documented. The majority of cases, however, are infected through venereal transmission or by contact with the fetus or fetal membranes after abortions and stillbirths. Puppies can be infected in utero, and may remain persistently infected even if they appear normal. Nursing puppies can also be infected from feeding on contaminated milk.

These bacteria have a wide geographical distribution. They can be spread on fomites and they can remain viable for several months in water, aborted fetuses, feces, equipment and clothing. Like other Brucella species, B. canis can withstand drying, particularly when organic material is present, and can survive in dust and soil for long periods.

B. canis is a gram-negative coccobacillus or short rod bacterium and is a facultative intracellular pathogen. It does not grow well in culture; overgrowth of cultures by other organisms often prevents its detection by this method. Serological detection of B. canis infection has a low specificity because the lipopolysaccharide surface (LPS) antigen on the surface of the B. canis bacteria, which is the common target of the serological detection method, is also found in several other bacterial species. However, molecular detection by polymerase chain reaction is highly specific, sensitive and fast. (Keid et al, 2007).

Utilities:

  • Help confirm the disease causing agent
  • Help identify Brucella canis carriers
  • Help ensure that dog kennels and shows are free of Brucella canis bacteria
  • Early prevention of spread of Brucella canis among dogs
  • Environmental testing to avoid reinfection
  • Safety monitoring of biological products that derive from susceptible animals

References:
Keid, L.B., Soares, R.M., Vieira, N.R., Megid, J., Salgado, V.R., Vasconcellos, S.A., da Costa, M., Gregori, F. and Richtzenhain, L.J. (2007) Diagnosis of canine brucellosis: comparison between serological and microbiological tests and a PCR based on primers to 16S-23S rDNA interspacer. Vet. Res. Commun 31:951- 965.

Specimen requirements: 0.2 ml fresh or frozen semen, or vaginal swab, or urethral swab, or prepuce swab, or 0.2 ml EDTA whole blood, or 0.2 ml urine, or 0.2 ml milk, or environmental surface swab; or swab or fetal tissue (fresh or frozen) from abortion or stillbirth.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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