NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

            * * *           

Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline pneunomitis

Feline rhinotracheitis virus

Feline syncytial virus

Francisella tularensis

Giardia

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Feline leukemia PCR test

dog and cat assay data sheet

 

Feline leukemia virus (FeLV)

Test code:
S0111 - Ultrasensitive qualitative detection of feline leukemia virus by real time polymerase chain reaction.  This assay detects but does not differentiate proviral DNA of feline leukemia virus types A, B and C in sample types containing cellular material.

Test code: S0171 - Ultrasensitive quantitative detection of feline leukemia virus proviral DNA by real time polymerase chain reaction.  This assay quantitates but does not differentiate feline leukemia virus types A, B and C in sample types containing cellular material.

Test code: S0172 - Ultrasensitive quantitative detection of feline leukemia virus RNA by real time polymerase chain reaction.  This assay quantitates but does not differentiate proviral DNA of feline leukemia virus types A, B and C in sample types containing cellular material.

S0111 is included onP0021 - feline bloodborne panel and on P0040 - feline anemia panel

 

Feline leukemia virus (FeLV) infection is one of the leading causes of death in cats. In the United States, 2% to 3% of all cats are infected with FeLV.

FeLV is a retrovirus which infects domestic cats and some wild felines. There are three types: FeLV-A, FeLV-B and FeLV-C. Cats can be infected with one, two or all three types simultaneously. Infection with FeLV-A can result in severe immunosuppression. When infected with FeLV-B, cats develop neoplastic disease (ie tumors and other abnormal tissue growths) more readily than cats infected only with FeLV-A. FeLV-C is seen in only about 1% of FeLV-infected cats and causes severe anemia.

After initial infection, the virus replicates in the tonsils and pharyngeal lymph nodes. It spreads via the bloodstream to other parts of the body, especially other lymph nodes, bone marrow and intestinal tissue, where it continues to replicate. Viremia, the presence of virus in the blood, usually begins 2 to 4 weeks after the initial infection.

There are several risk factors for FeLV infection. Ill cats are four times more likely than healthy cats to become infected with FeLV. Researchers estimate that about 50% of cats with severe bacterial infections and 75% of cats with toxoplasmosis also have FeLV infections. Outdoor cats are more likely than indoor cats to be infected with FeLV. Less than 1% of healthy indoor cats in the United States are infected with FeLV, compared to 1% to 2% of healthy outdoor cats, and more than 13% of ill stray cats.

FeLV can easily be transmitted via saliva. It can also spread through infected urine, tears and feces, and to kittens during gestation and nursing. Twenty percent of FeLV-positive mothers pass the virus to their kittens. Other modes of transmission include bites from infected cats, blood transfusions, mutual grooming, nose-to-nose contact, shared food dishes and water bowls, shared litter trays and sneezing.

FeLV vaccination does not provide absolute protection against FeLV infection, so chronically ill cats should be tested for FeLV even if vaccinated.

Serological diagnosis of FeLV has been used to diagnose FeLV-infected cats. However, cats with weakened immune response due to other infections or diseases will not be reliably detected by serological methods. Molecular detection by PCR overcomes this problem, because PCR detects the presence of the pathogen itself rather than the host animal’s immune response. PCR is rapid, highly sensitive and specific. A recent study (Gomes-Keller et al., 2006) has shown that FeLV proviral DNA detection in blood by PCR provides significantly higher sensitivity, specificity and positive and negative predictive values than serology.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of FeLV infection
  • Help ensure that feline populations are free of FeLV
  • Early prevention of spread of this virus among a feline population
  • Quantitative measurement of FeLV RNA or FeLV proviral DNA in a sample
  • Minimize human exposure to this virus

Reference:
Gomes-Keller, M.A., Gönczi, E., Tandon, R., Riondato, F., Hofmann-Lehmann, R.,Meli, M.L. and Lutz, H. (2006) Detection of feline leukemia virus RNA in saliva from naturally infected cats and correlation of PCR results with those of current diagnostic methods. J. Clin. Microbiol. 44:916-22.

Specimen requirement: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodologies:
S0111: Qualitative real time PCR targeting FeLV cellular proviral DNA

S0171: Quantitative real time PCR targeting FeLV cellular proviral DNA
S0172: Quantitative real time PCR targeting FeLV cellular RNA

Normal range: Nondetected

©2003-2014 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880