NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Fading kitten syndrome

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Group G strep

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leishmania

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus, Group G

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Canine enteric coronavirus PCR test

dog and cat assay data sheet

 

Canine enteric coronavirus (CCV1 or CCoV1)

Test code: S0107 - Ultrasensitive qualitative detection of canine enteric coronavirus (type 1) by reverse transcription coupled real time polymerase chain reaction.  This assay does not detect canine respiratory coronavirus (type 2).

S0107 is included on P0022 - canine diarrhea panel

 

Canine coronavirus type 1 (CCV1) causes sporadic outbreaks of enteritis in dogs. Although dogs of all ages are susceptible to canine coronavirus, young pups are more prone to develop clinical symptoms. The normal route of transmission is fecal-oral. Infected dogs can release CCV1 in their feces for 6-9 days, but shedding can be prolonged in some pups even after clinical signs have disappeared.  CCV1 is genetically distinct from the group 2 respiratory coronavirus that can cause respiratory disease in dogs.

The virus is highly contagious. CCV1 is resistant to acidic conditions, passing through the stomach without any damage. The surface epithelium of the small intestine is the main target of CCV1, while the colon is resistant to the infection.

Incubation time is very short once dogs are infected. Vomiting and diarrhea start to develop 1-3 days post infection and are followed by full-blown symptoms. Feces may be mucoid or watery, sometimes streaked with blood, and the feces are exceptionally malodorous. Infected pups can quickly become dehydrated even if fluid therapy is started early, and they may become depressed and anorexic. While the infection is generally afebrile, elevated body temperature has been observed in some cases.

Secondary infections by bacteria, parasites or other viruses such as parvoviruses or rotaviruses develop easily and can cause prolongation of illness. Dogs usually recover spontaneously from CCV1 within one week, but illness sometimes lasts two weeks or longer. The mortality rate for canine coronavirus infection alone is usually very low, but deaths have been reported in some kennels, especially in pups.

Canine coronavirus-induced enteritis is very difficult to differentiate from enteritis caused by other agents. Clinical testing is important to correctly identify the pathogen. Assays which have been used for the detection of CCV1 in fecal samples include electron microscopy (EM) and isolation on appropriate cell cultures. However, detection by EM is very expensive and labor intensive and is not available in most clinical testing laboratories. Cell culture is neither very specific nor very sensitive, and requires delicate care in handling and shipping specimens. On the other hand, molecular detection by PCR offers a rapid, sensitive and specific method for identifying CCV1, and specimens require less stringent handling.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of CCV1 infection
  • Help ensure that canine populations are free of CCV1
  • Early prevention of spread of this virus among a canine population
  • Minimize human exposure to this virus

Specimen requirements: 0.2 ml feces or rectal swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

Canine enteric coronavirus PCR test

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