NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline pneunomitis

Feline rhinotracheitis virus

Feline syncytial virus

Francisella tularensis

Giardia

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Streptococcus zooepidemicus PCR test for dogs

dog and cat assay data sheet

"Strep zoo" in dogs (hemorrhagic streptococcal pneumonia)

Test code:
B0019 - Qualitative detection of Streptococcus equi subspecies zooepidemicus by polymerase chain reaction. This assay detects and differentiates subspecies equi and subspecies zooepidemicus.

 

Streptococcus equi subsp. equi is the etiological agent of strangles and is responsible for nearly 30% of all reported equine infections worldwide (Chanter, 1997). The very closely related organism Streptococcus zooepidemicus (S. equi subsp. zooepidemicus) has also been found to be a significant cause of equine lower airway disease, foal pneumonia, endometritis, and abortion (Chanter, 1997). In dogs, S. zooepidemicus is associated with hemorrhagic streptococcal pneumonia (HSP) (Garnett et al., 1982). The HSP syndrome is a severe infection, in which sudden death can occur without any prior clinical signs. In general, dogs with higher S. equi subsp. zooepidemicus scores of infection were more likely to have severe alveolar damage (Chalker et al., 2003).

In the past, identification of S. equi bacteria usually relied on culture of the bacteria, but this technique is slow and not very sensitive. A recent study (Newton, 2000) has shown that repeated nasopharyngeal swabbing and culture of Streptococcus equi could not detect the development of healthy carriers in more than 50% of equine strangles outbreaks. S. equi was sometimes not detected by culture of nasopharyngeal swabs from carriers for up to 2 or 3 months before nasal shedding resumed sporadically. The study found that PCR was a more sensitive technique for detecting S. equi on swabs: many more known positive swabs were detected using PCR than using culture (56 of 61 swabs positive by PCR vs. 18 of 61 swabs positive by culture). Similar results were obtained for equine guttural pouch samples from 12 established carriers (PCR 76% vs. culture 59%). PCR also allows differentiation of the two subspecies, equi and zooepidemicus.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that dog populations are free of Streptococcus zoopidemicus
  • Early prevention of spread of Strep zoo among a group of dogs
  • Minimize human exposure to Strep zoo
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Chalker, V.J., Brooks, H.W. and Brownlie, J. (2003) The association of Streptococcus equi subsp. zooepidemicus with canine infectious respiratory disease. Vet Microbiol. 95:149-156.
Chanter, N. (1997) Streptococci and enterococci as animal pathogens. J. Appl. Microbiol. Symp. Suppl. 83:100S-109S.
Garnett, N.L., Eydelloth, R.S., Swindle, M.M., Vonderfecht, S.L., Strandberg, J.D. and Luzarraga, M.B. (1982) Hemorrhagic streptococcal pneumonia in newly procured research dogs. J. Am. Vet. Med. Assoc. 181:1371–1374.
Newton, J.R., Verheyen, K., Talbot, N.C., Timoney, J.F., Wood, J.L., Lakhani, K.H. and Chanter, N. (2000) Control of strangles outbreaks by isolation of guttural pouch carriers identified using PCR and culture of Streptococcus equi. Equine Vet J. 32:515-526.

Canine specimen requirement: Nasopharyngeal swab, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative PCR

Normal range: Nondetected

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