NEW - Neuro symptoms getting on your nerves? Try our canine neurological panel - 6 neurological pathogens from 1 CSF sample; or our feline neurological panel - 5 neurological pathogens from 1 CSF sample.

Oh baby! Try our canine breeding PCR panel - 3 canine sexually transmitted diseases tested from swabs or semen samples.

Respiratory symptoms got you breathless? Try our canine respiratory PCR panel - we test for 6 canine respiratory pathogens from throat, nasal and eye swabs.

...or maybe you need our feline respiratory PCR panel -- 6 feline respiratory pathogens from throat, nasal and eye swabs.

Diarrhea got you on the run? Try our canine diarrhea PCR panel -- 8 major diarrheagenic agents from 1 fecal specimen...
...OR our 9-pathogen feline diarrhea PCR panel.

Not feeling sanguine about bloodborne pathogens in cats? Try our feline bloodborne PCR panel -- 4 major bloodborne pathogens from 1 blood sample.

Ticks bugging you? Try our tickborne disease PCR panel -- 7 major tickborne pathogens from 1 blood sample.

Just plain sick and tired? Try our canine anemia PCR panel or our feline anemia PCR panel -- detect and differentiate multiple anemia pathogens from 1 blood sample.

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Zoologix performs canine and feline PCR tests for...

Anaplasma phagocytophilum

Anaplasma platys

Aspergillus species

Aspergillus fumigatus

Babesia

Bartonella

Baylisascaris procyonis

Bordetella bronchiseptica

Borrelia burgdorferi

Brucella

Campylobacter

Canine adenovirus type 1

Canine adenovirus type 2

Canine enteric coronavirus (CCV1)

Canine distemper

Canine herpesvirus

Canine papillomavirus

Canine parainfluenza virus

Canine parvovirus

Canine respiratory coronavirus (CCV2)

Chagas disease

Chikungunya virus

Chlamydophila psittaci

Clostridium species

Coccidia

Cryptococcus

Cryptosporidium

Cytauxzoon felis

E. coli

Ehrlichia

Feline calicivirus

Feline distemper

Feline enteric coronavirus

Feline foamy virus

Feline herpesvirus type 1

Feline immunodeficiency virus

Feline infectious anemia

Feline infectious peritonitis

Feline leukemia

Feline panleukopenia

Feline papillomavirus

Feline pneunomitis

Feline rhinotracheitis virus

Feline sarcoma virus

Feline syncytial virus

Francisella tularensis

Giardia

Haemobartonella canis

Haemobartonella felis

Helicobacter

Influenza

Lawsonia intracellularis

Leptospira

Lyme disease

MRSA (Methicillin-resistant Staph aureus)

Mycoplasma canis

Mycoplasma felis

Mycoplasma haemocanis

Mycoplasma haemofelis

Neospora caninum

Pasteurella multocida

Pneumocystis carinii

Rabies

Reovirus screen

Rickettsia screen

Salmonella

Sarcocystis neurona

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus zooepidemicus

Toxoplasma gondii

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Tularemia

West Nile virus

Yersinia pestis

Yersinia pseudotuberculosis


Distemper PCR test

dog and cat assay data sheet

Canine distemper virus (CDV)

Test code:
S0092 - Ultrasensitive qualitative detection of canine distemper virus by reverse transcription coupled real time polymerase chain reaction

S0092 is included on P0036 - canine neurological panel, and on P0019 - canine respiratory panel

Canine distemper (CD) is a highly contagious disease in young dogs, particularly those 3 to 6 months of age. It has a high morbidity and mortality rate. The disease can be spread by aerosol infection (Appel and Gillespie, 1972) and is characterized by a diphasic fever curve and acute rhinitis, and later by bronchitis, catarrhal pneumonia, severe gastroenteritis, and nervous signs.

The causative agent of the disease is a virus belonging to the genus Morbillivirus of family Paramyxoviridae. Since the canine distemper virus (CDV) can survive for a longer period of time in cold conditions, the disease spreads mainly in winter months. Although the disease is highly communicable, it is comparatively rare in many developed countries due to vaccination using the attenuated live virus, but occasional outbreaks of CDV infection can still occur in vaccinated populations of dogs. In areas with unvaccinated populations, CD is extremely widespread.

The host spectrum of CDV comprises dogs and many other carnivores and noncarnivores as well as marine mammals. Recently, a possible link between Paget's disease of bone in humans and CDV infection was shown by epidemiological studies and was substantiated by detection of CDV RNA in affected tissues (Gordon, et al., 1992; O’Driscoll, et al., 1990). CDV is also discussed as a candidate that might play a role in the initiation of multiple sclerosis (Rohowsky-Kochan, et al., 1995). Thus prevention of CDV infection in house dogs may have a direct impact on human safety.

Diagnosis of CD in acute or subacute form is usually based on clinical signs and history in unvaccinated puppies. But it has been difficult to differentiate CD from other diseases such as kennel cough in the early stage. Serologic detection of IgM antibody can be useful, but poses a problem in young puppies due to uncertainty caused by maternal antibody interference. Definitive diagnosis can be made through isolation of CDV, or through detection of CDV in epithelial cells after fluorescent antibody (FA) staining. However, virus isolation takes several days to weeks and is frequently not effective in the acute stage of the infection. In addition, FA testing is successful only during the first few days of acute signs of distemper.

CDV detection by PCR is the most rapid, sensitive and specific method for the diagnosis of this infection. It also helps to eliminate false negative and positive cases.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal groups and populations are free of CDV
  • Early prevention of spread of this virus among a population
  • Minimize human exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Appel, M. J. G., and Gillespie, J.H.(1972). Canine distemper virus, p. 1-96. In S. Gard, C. Hallauer, and K. F. Meyer (ed.), Virology monographs 11. Springer-Verlag, New York, N.Y.
Gordon, M. T., Mee, A.P., Anderson, D.C. and Sharp, P.T. (1992) Canine distemper virus transcripts sequenced from pagetic bone. Bone Miner. 19:159-174.
O'Driscoll, J. B., Buckler, H.M., Jeacock, J. and Anderson, D.C. (1990) Dogs, distemper and osteitis deformans: a further epidemiological study. Bone Miner. 11:209-216.
Rohowsky-Kochan, C., Dowling, P.C., and Cook, S.D. (1995) Canine distemper virus-specific antibodies in multiple sclerosis. Neurology 45:1554-1560.

Specimen requirement: Nasopharyngeal swab, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml CSF, urine, plasma or serum, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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