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rodent and rabbit assay data sheet
Pneumonia virus of mice (PVM)
Test code:
S0139 -
Ultrasensitive qualitative detection of pneumonia virus of mice
by reverse transcription coupled real time PCR
S0139 is included on
P0029 - Mouse Essentials Panel
Pneumonia virus
of mice (PVM) belongs to the family Paramyxoviridae, subfamily
Pneumonvirinae, genus
Pneumovirus.
Other members of Pneumovirus include human and bovine syncytial
viruses (HRSV and BRSV, respectively).
Infection of
laboratory rodents by PVM is widespread because clinical signs
of disease and gross lesions are absent in infections of
immunocompetent rodents, making carriers difficult to detect.
(Cook et al., 1998).
PVM infection of immunodeficient rodents, such as nude mice, may
cause a persistent wasting disease syndrome.
Serological
detection can identify rodents previously exposed to PVM, but
serology is not a good tool to identify current carriers of this
virus. Molecular detection by PCR is the most sensitive,
specific and rapid means to identify these carriers.
Utilities:
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Help confirm the disease causing agent
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Shorten the time required to confirm a clinical
diagnosis of PVM
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Help ensure that vivariums are free of PVM
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Early prevention of spread of this virus among a
vivarium
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Minimize personnel exposure to this virus
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Safety monitoring of biological products that derive
from mice
References:
Cook, P.M., Eglin, R.P. and Easton, A.J. (1998) Pathogenesis of
pneumovirus infections in mice: detection of pneumonia virus of
mice and human respiratory syncytial virus mRNA in lungs of
infected mice by in situ
hybridization. J. Gen. Virol. 79: 2411-2417.
Specimen requirements:
0.2 ml whole blood in EDTA (purple top) tube,
or 0.2 ml cell culture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
For all
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
shipping instructions
for more information.
Turnaround time:
2 business days
Methodology:
Qualitative
reverse transcription coupled real time polymerase chain
reaction
Normal range: Nondetected
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