Screening your mice? Try our Mouse Essentials PCR Panel. All the most important mouse colony screening tests, all by expert real time PCR...

...or how about our new Mouse PCR Minipanel - PCR tests for only the most common mouse pathogens - for economical colony screening...

...and don't forget our Mouse Fecal PCR Panel - includes 9 important fecal pathogens.

And... just for rabbits: our new Rabbit Fecal PCR Panel tests for 3 common causes of GI problems in rabbits.

For wild rodent infestations, remediation and environmental monitoring, use our Rodent Infestation PCR Panel

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Zoologix also performs rodent and rabbit PCR tests for...

Aspiculuris tetraptera


BXV-1 virus



Clostridium piliforme


E. coli (enteroinvasive)



Encephalitozoon cuniculi


Francisella tularensis

Fur mites



Human adenoviruses

Klebsiella pneumoniae

K virus

Lactate dehydrogenase-elevating virus


Lymphocytic choriomeningitis virus (LCMV)


Mouse adenoviruses

Mouse cytomegaloviruses

Mouse hepatitis virus (MHV)

Mouse kidney parvovirus (MKV or MKPV)

Mouse minute virus (MMV)

Mouse norovirus (MNV)

Mouse parvovirus (MPV)

Mouse polyoma virus (POLY)

Mousepox virus (aka ectromelia virus, EV or ECTRO)

Mouse rotavirus

Mycoplasma pulmonis

Mycoplasma screen



Pneumocystis carinii

Pneumonia virus of mice (PVM)

Rabbit coronavirus

Rabbit fibroma virus

Rabbit hemorrhagic disease virus

Rat bite fever

Rat coronavirus

Reovirus screen

Reovirus type 3 (REO3)



Sendai virus (SEND)

Seoul virus


Sialodacryoadenitis virus (SDAV)

Streptobacillus moniliformis

Streptococcus pneumoniae

Syphacia muris

Syphacia obvelata

Theiler's murine encephalomyelitis virus (TMEV)

Tickborne encephalitis virus

Treponema cuniculi/ paraluiscuniculi


Tyzzer's disease

Whitewater Arroyo virus

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis

Mycoplasma PCR tests for rodents

rodent and rabbit assay data sheet


Test codes:

B0002 - Qualitative detection of Mycoplasma pneumoniae by polymerase chain reaction.

B0009 - Ultrasensitive Mycoplasma screen by real time PCR.  This screen detects but does not differentiate M. arginini, M. fermentans, M. hominis, M. hyorhinis, M. orale, M. pirum, M. salivarium, M. agassizii, M. cynos and others. This screen does not detect M. pneumoniae or M. pulmonis.

B0041 - Ultrasensitive qualitative detection of Mycoplasma pulmonis by real time polymerase chain reaction

 B0041 is included on P0029 - Mouse Essentials Panel

Murine respiratory mycoplasmosis in mice and rats is caused by Mycoplasma pulmonis. In addition to exhibiting high morbidity and reduced birth rate, mice and rats infected with M. pulmonis often develop imperceptible infections. These can cause problems in research studies using these animals, as physiological mechanisms and the immune system may be affected. Experimental results obtained with infected animals can be misleading.

Diagnosis of M. pulmonis infection can be done using serological methods such as microbial isolation or enzyme linked immunosorbent assay (ELISA) (Cassell et al., 1981). However, in vitro isolation is time-consuming, and serological methods often give incorrect results due to cross-reactivity between different species of rodent mycoplasmas (Cassell et al., 1981; Davis et al., 1987; Davidson et al., 1994). Serological assays also lack sensitivity because a low level of antibody -- early in an infection or in immunodeficient animals -- may not be detected.

Detection of this pathogen by polymerase chain reaction is especially important because it is very sensitive and specific. The result will not be compromised if the infection is at an early stage or the animal is immunodeficient.


  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of M. pulmonis infection.
  • Help ensure that rodent colonies are free of M. pulmonis
  • Early prevention of spread of M. pulmonis among a colony
  • Minimize personnel exposure to M. pulmonis
  • Safety monitoring of biological products that derive from rodents

Cassell, G.H., Lindsey, J.R., Davis, J.K., Davidson, M.K., Brown, M.B. and Mayo, J.G. (1981) Detection of natural Mycoplasma pulmonis infection in rats and mice by an enzyme linked immunosorbent assay (ELISA). Lab Anim Sci 31: 676–682.
Davis, J.K., Cassell, G.H., Gambill, G., Cox, N., Watson, H. and Davidson, M. (1987) Diagnosis of murine mycoplasmal infections by enzyme-linked immunosorbent assay (ELISA). Isr J Med Sci 23: 717–722.
Davidson, M.K, Davis, J.K., Gambill, G.P., Cassell, G.H. and Lindsey, J.P. (1994) Mycoplasmas of laboratory rodentsMycoplasmas in Animals: Laboratory Diagnosis, Iowa State University Press, Ames p. 97–133.

Specimen requirements:

B0009 - 0.2 ml whole blood in EDTA (purple top) tube, or 0.2 ml fresh, frozen or fixed tissue, or nasal swab, or vaginal swab, or 0.2 ml bacterial culture or cell culture.

B0002 or B0041 - Nasal swab, or 0.2 ml bacterial culture or cell culture.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative PCR (B0002); qualitative real time PCR (B0009 and B0041)

Normal range: Nondetected

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