rodent and rabbit assay data sheet
- Qualitative detection of Mycoplasma
pneumoniae by polymerase chain reaction.
Ultrasensitive Mycoplasma screen by real time PCR. This
screen detects but does not differentiate M. arginini, M.
fermentans, M. hominis, M. hyorhinis, M. orale, M. pirum, M.
salivarium, M. agassizii, M. cynos and others. This screen does
not detect M. pneumoniae or M. pulmonis.
- Ultrasensitive qualitative detection of
by real time polymerase chain reaction
B0041 is included on P0029 -
Mouse Essentials Panel
respiratory mycoplasmosis in mice and rats is caused by
In addition to exhibiting high morbidity and reduced birth rate,
mice and rats infected with
M. pulmonis often develop imperceptible infections.
These can cause problems in research studies using these
animals, as physiological mechanisms and the immune system may
be affected. Experimental results obtained with infected animals
can be misleading.
infection can be done using serological methods such as
microbial isolation or enzyme linked immunosorbent assay (ELISA)
(Cassell et al., 1981). However, in vitro isolation is
time-consuming, and serological methods often give incorrect
results due to cross-reactivity between different species of
rodent mycoplasmas (Cassell et al., 1981; Davis et al., 1987;
Davidson et al., 1994). Serological assays also lack sensitivity
because a low level of antibody -- early in an infection or in
immunodeficient animals -- may not be detected.
this pathogen by polymerase chain reaction is especially
important because it is very sensitive and specific. The result
will not be compromised if the infection is at an early stage or
the animal is immunodeficient.
Help confirm the disease causing agent
Shorten the time required to confirm a clinical
diagnosis of M. pulmonis
Help ensure that rodent colonies are free of
Early prevention of spread of
among a colony
Minimize personnel exposure to
Safety monitoring of biological products that derive
Cassell, G.H., Lindsey, J.R., Davis, J.K., Davidson, M.K.,
Brown, M.B. and Mayo, J.G. (1981) Detection of natural
Mycoplasma pulmonis infection in rats and mice by an enzyme
linked immunosorbent assay (ELISA). Lab Anim Sci 31: 676–682.
Davis, J.K., Cassell, G.H., Gambill, G., Cox, N., Watson, H. and
Davidson, M. (1987) Diagnosis of murine mycoplasmal infections
by enzyme-linked immunosorbent assay (ELISA). Isr J Med Sci 23:
Davidson, M.K, Davis, J.K., Gambill, G.P., Cassell,
G.H. and Lindsey, J.P. (1994) Mycoplasmas of laboratory
rodentsMycoplasmas in Animals: Laboratory Diagnosis, Iowa State
University Press, Ames p. 97–133.
- 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top)
tube, or 0.2 ml fresh, frozen or fixed tissue, or nasal swab, or
vaginal swab, or 0.2 ml bacterial culture or cell culture.
B0002 or B0041
- Nasal swab,
or 0.2 ml bacterial culture or cell culture.
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See shipping
instructions for more information.
2 business days
PCR (B0002); qualitative real time PCR (B0009 and B0041)